Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The dynamics of centromere motion through the metaphase-to-anaphase transition reveal a centromere separation order

Jonathan W. Armond, Katie L. Dale, Nigel J. Burroughs, Andrew D. McAinsh, Elina Vladimirou
doi: https://doi.org/10.1101/582379
Jonathan W. Armond
1Department of Oncology, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6DD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katie L. Dale
1Department of Oncology, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6DD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nigel J. Burroughs
2Warwick Systems Biology Centre and Mathematics Institute, University of Warwick, Coventry, CV4 7AL UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew D. McAinsh
3Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elina Vladimirou
1Department of Oncology, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6DD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: e.vladimirou@ucl.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

During cell division, chromosomes align at the equator of the cell before sister chromatids separate to move to each daughter cell during anaphase. We use high-speed imaging, Bayesian modelling and quantitative analysis to examine the regulation of centromere dynamics through the metaphase-to-anaphase transition. We find that, contrary to the apparent instantaneous separation seen in low-frequency imaging, centromeres separate asynchronously over 1-2 minutes. The timing of separations negatively correlates with the centromere intersister distance during metaphase, which could potentially be explained by variable amounts of cohesion at centromeres. Depletion of condensin I increases this asynchrony. Depletion of condensin II, on the other hand, abolishes centromere metaphase oscillations and impairs centromere speed in anaphase. These results suggest that condensin complexes have broader direct roles in mitotic chromosome dynamics than previously believed and may be crucial for the regulation of chromosome segregation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted March 19, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The dynamics of centromere motion through the metaphase-to-anaphase transition reveal a centromere separation order
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The dynamics of centromere motion through the metaphase-to-anaphase transition reveal a centromere separation order
Jonathan W. Armond, Katie L. Dale, Nigel J. Burroughs, Andrew D. McAinsh, Elina Vladimirou
bioRxiv 582379; doi: https://doi.org/10.1101/582379
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
The dynamics of centromere motion through the metaphase-to-anaphase transition reveal a centromere separation order
Jonathan W. Armond, Katie L. Dale, Nigel J. Burroughs, Andrew D. McAinsh, Elina Vladimirou
bioRxiv 582379; doi: https://doi.org/10.1101/582379

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2420)
  • Biochemistry (4779)
  • Bioengineering (3319)
  • Bioinformatics (14637)
  • Biophysics (6620)
  • Cancer Biology (5158)
  • Cell Biology (7405)
  • Clinical Trials (138)
  • Developmental Biology (4343)
  • Ecology (6864)
  • Epidemiology (2057)
  • Evolutionary Biology (9893)
  • Genetics (7331)
  • Genomics (9502)
  • Immunology (4540)
  • Microbiology (12644)
  • Molecular Biology (4928)
  • Neuroscience (28245)
  • Paleontology (199)
  • Pathology (803)
  • Pharmacology and Toxicology (1384)
  • Physiology (2014)
  • Plant Biology (4482)
  • Scientific Communication and Education (975)
  • Synthetic Biology (1295)
  • Systems Biology (3907)
  • Zoology (722)