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Sumoylation regulates central spindle protein dynamics during chromosome segregation in oocytes

View ORCID ProfileFederico Pelisch, Laura Bel Borja, Ellis G. Jaffray, View ORCID ProfileRonald T. Hay
doi: https://doi.org/10.1101/584763
Federico Pelisch
aCentre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee. Dundee, DD1 5EH, United Kingdom
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  • For correspondence: f.pelisch@dundee.ac.uk
Laura Bel Borja
aCentre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee. Dundee, DD1 5EH, United Kingdom
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Ellis G. Jaffray
aCentre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee. Dundee, DD1 5EH, United Kingdom
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Ronald T. Hay
aCentre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee. Dundee, DD1 5EH, United Kingdom
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Abstract

Meiotic spindles in most species lack centrosomes and the mechanisms that underlie faithful chromosome segregation in acentrosomal meiotic spindles are not well understood. In C. elegans oocytes, spindle microtubules exert a poleward force on chromosomes dependent on the microtubule-stabilising protein CLS-2CLASP. The kinase BUB-1Bub1 and CLS-2CLASP localise in the central-spindle and display a dynamic localisation pattern throughout anaphase but the signals regulating their anaphase-specific localisation remains unknown. We have shown that SUMO regulates BUB-1 localisation during metaphase I. Here, we found that SUMO modification of BUB-1Bub1 is regulated by the SUMO E3 ligase GEI-17 and the SUMO protease ULP-1. SUMO is required for BUB-1 localisation in between segregating chromosomes during early anaphase I, and SUMO depletion partially phenocopies BUB-1Bub1 depletion. We also show that CLS-2CLASP is subject to SUMO-mediated regulation. Over-all, we provide evidence for a novel, SUMO-mediated control of protein dynamics during early anaphase I in oocytes.

  • meiosis
  • oocytes
  • SUMO
  • chromosomes
  • segregation
  • central-spindle
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted March 27, 2019.
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Sumoylation regulates central spindle protein dynamics during chromosome segregation in oocytes
Federico Pelisch, Laura Bel Borja, Ellis G. Jaffray, Ronald T. Hay
bioRxiv 584763; doi: https://doi.org/10.1101/584763
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Sumoylation regulates central spindle protein dynamics during chromosome segregation in oocytes
Federico Pelisch, Laura Bel Borja, Ellis G. Jaffray, Ronald T. Hay
bioRxiv 584763; doi: https://doi.org/10.1101/584763

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