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Differential impacts of the head on Platynereis dumerilii peripheral circadian rhythms

View ORCID ProfileEnrique Arboleda, Martin Zurl, View ORCID ProfileKristin Tessmar-Raible
doi: https://doi.org/10.1101/593772
Enrique Arboleda
1Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/4, A-1030 Vienna
3Institut de Génomique Fonctionnelle de Lyon (IGFL), École Normale Supérieure de Lyon, 32 avenue Tony Garnier, 69007 Lyon, France
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Martin Zurl
1Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/4, A-1030 Vienna
2Research Platform “Rhythms of Life”, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/4, A-1030 Vienna
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Kristin Tessmar-Raible
1Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/4, A-1030 Vienna
2Research Platform “Rhythms of Life”, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9/4, A-1030 Vienna
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  • For correspondence: kristin.tessmar@mfpl.ac.at
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Abstract

Background The marine bristle worm Platynereis dumerilii is a useful functional model system for the study of the circadian clock and its interplay with others, e.g. circalunar clocks. The focus has so far been on the worm’s head. However, behavioral and physiological cycles in other animals typically arise from the coordination of circadian clocks located in the brain and in peripheral tissues. Here we focus on peripheral circadian rhythms and clocks, revisit and expand classical circadian work on the worm’s chromatophores, investigate locomotion as read-out and include molecular analyses.

Results We establish that different pieces of the trunk exhibit synchronized, robust oscillations of core circadian clock genes. These circadian core clock transcripts are under strong control of the light-dark cycle, quickly losing synchronized oscillation under constant darkness, irrespective of the absence or presence of heads. Different wavelengths are differently effective in controlling the peripheral molecular synchronization. We have previously shown that locomotor activity is under circadian clock control. Here we show that upon decapitation it still follows the light-dark cycle, but does not free-run under constant darkness. We also observe the rhythmicity of pigments in the worm’s individual chromatophores, confirming that chromatophore size changes follow a circadian pattern. These size changes continue under constant darkness, but cannot be re-entrained by light upon decapitation.

Conclusions Here we provide the first basic characterization of the peripheral circadian clock of Platynereis dumerilii. In the absence of the head, light is essential as a major synchronization cue for peripheral molecular and locomotor circadian rhythms. Circadian changes in chromatophore size can however continue for several days in the absence of light/dark changes and the head. Thus, the dependence on the head depends on the type of peripheral rhythm studied. These data show that peripheral circadian rhythms and clocks should be considered when investigating the interactions of clocks with different period lengths, a notion likely also true for other organisms with circadian and non-circadian clocks.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 30, 2019.
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Differential impacts of the head on Platynereis dumerilii peripheral circadian rhythms
Enrique Arboleda, Martin Zurl, Kristin Tessmar-Raible
bioRxiv 593772; doi: https://doi.org/10.1101/593772
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Differential impacts of the head on Platynereis dumerilii peripheral circadian rhythms
Enrique Arboleda, Martin Zurl, Kristin Tessmar-Raible
bioRxiv 593772; doi: https://doi.org/10.1101/593772

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