Abstract
Soil-borne pathogens are serious problems for crop production. Chemical-dependent soil fumigation method has been an effective method for controlling soil-borne pathogens, but it is escaped for food safety and environmental pollution. Anaerobic soil disinfestation (ASD) is a chemical-independent fumigation method and can reduce pathogens under 40 cm soil depth. However, their effect is not stable under the field condition. Soil microbes have important roles for ASD. Microbial community reflects the soil environment and some unique microbes are be detected under different condition. If common microbes are shown in well-disinfested multiple fields, they will be a good indicator for the success of ASD. For this purpose, we conducted the same ASD treatment with 17 tomato greenhouses where tomato bacterial wilt occurred in the geographically different areas. In this study, we compared soil prokaryotic communities in the field which indicate different disinfestation effects after ASD treatment among several fields using the next-generation sequencer. Weighted-UniFrac analysis showed that prokaryotic communities in the fields showing different disinfestation effect were not different and they were roughly separated to sampling fields. The relative abundance of Betaproteobacteria and Clostridia were significantly increased in well- disinfested fields. Indicator species analysis revealed that twenty-five operational taxonomic units (OTUs) were specifically detected from various well-disinfested soil and 18 OTUs belonged to phylogenetically diversified Clostridia. Other OTUs belonged to aerobic bacteria and were not previously detected from ASD treated fields. Our results showed that the prokaryotic communities were not largely differed by ASD efficiency but small changing will be important for ASD.
Abbreviations
- ASD
- Anaerobic soil disinfestation
- OTU
- Operation taxonomic units
- PCR
- Polymerase chain reaction