Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose; Nicholas A. Popp; Christopher D. Richardson; Jason J. Stephany; Julie Mathieu; Cindy T. Wei; Jacob E. Corn; Dustin J. Maly; Douglas M. Fowler

doi:10.1101/597849

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Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

View ORCID ProfileJohn C. Rose, View ORCID ProfileNicholas A. Popp, Christopher D. Richardson, Jason J. Stephany, Julie Mathieu, Cindy T. Wei, Jacob E. Corn, View ORCID ProfileDustin J. Maly, View ORCID ProfileDouglas M. Fowler

doi: https://doi.org/10.1101/597849

John C. Rose

1Department of Chemistry, University of Washington, Seattle, Washington 98195 U.S.A.

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For correspondence: jackrose@uw.edu dfowler@uw.edu djmaly@uw.edu

Nicholas A. Popp

2Department of Genome Sciences, University of Washington, Seattle, Washington 98195 U.S.A.

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Christopher D. Richardson

3Innovative Genomics Initiative, University of California, Berkeley, Berkeley, California, 94720, U.S.A.

4Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, 94720, U.S.A.

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Jason J. Stephany

2Department of Genome Sciences, University of Washington, Seattle, Washington 98195 U.S.A.

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Julie Mathieu

5Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, 98109, U.S.A.

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Cindy T. Wei

1Department of Chemistry, University of Washington, Seattle, Washington 98195 U.S.A.

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Jacob E. Corn

3Innovative Genomics Initiative, University of California, Berkeley, Berkeley, California, 94720, U.S.A.

4Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, 94720, U.S.A.

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Dustin J. Maly

1Department of Chemistry, University of Washington, Seattle, Washington 98195 U.S.A.

6Department of Biochemistry, University of Washington, Seattle, Washington 98195 U.S.A.

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For correspondence: jackrose@uw.edu dfowler@uw.edu djmaly@uw.edu

Douglas M. Fowler

2Department of Genome Sciences, University of Washington, Seattle, Washington 98195 U.S.A.

7Department of Bioengineering, University of Washington, Seattle, Washington, 98195, U.S.A.

8Genetic Networks Program, Canadian Institute for Advanced Research, Toronto, Canada

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For correspondence: jackrose@uw.edu dfowler@uw.edu djmaly@uw.edu

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Posted April 03, 2019.

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Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose, Nicholas A. Popp, Christopher D. Richardson, Jason J. Stephany, Julie Mathieu, Cindy T. Wei, Jacob E. Corn, Dustin J. Maly, Douglas M. Fowler

bioRxiv 597849; doi: https://doi.org/10.1101/597849

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Suppression of unwanted CRISPR/Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

John C. Rose, Nicholas A. Popp, Christopher D. Richardson, Jason J. Stephany, Julie Mathieu, Cindy T. Wei, Jacob E. Corn, Dustin J. Maly, Douglas M. Fowler

bioRxiv 597849; doi: https://doi.org/10.1101/597849

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