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Ribo-seQC: comprehensive analysis of cytoplasmic and organellar ribosome profiling data

View ORCID ProfileLorenzo Calviello, View ORCID ProfileDominique Sydow, Dermot Harnett, Uwe Ohler
doi: https://doi.org/10.1101/601468
Lorenzo Calviello
1Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Hannoversche Strasse 28, Berlin 10115, Germany
2Departments of Biology and Computer Science, Humboldt-Universität zu Berlin, Unter den Linden 6, Berlin 10117, Germany
3Present address: Department of Cell and Tissue Biology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, 94143, California, USA
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Dominique Sydow
1Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Hannoversche Strasse 28, Berlin 10115, Germany
2Departments of Biology and Computer Science, Humboldt-Universität zu Berlin, Unter den Linden 6, Berlin 10117, Germany
4Present address: Institute of Physiology, Charité – Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany
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Dermot Harnett
1Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Hannoversche Strasse 28, Berlin 10115, Germany
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Uwe Ohler
1Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Hannoversche Strasse 28, Berlin 10115, Germany
2Departments of Biology and Computer Science, Humboldt-Universität zu Berlin, Unter den Linden 6, Berlin 10117, Germany
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  • For correspondence: [email protected]
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Abstract

Summary Ribosome profiling enables genome-wide analysis of translation with unprecedented resolution. We present Ribo-seQC, a versatile tool for the comprehensive analysis of Ribo-seq data, providing in-depth insights on data quality and translational profiles for cytoplasmic and organelle ribosomes. Ribo-seQC automatically generates platform-independent HTML reports, offering a detailed and easy-to-share basis for collaborative Ribo-seq projects.

Availability Ribo-seQC is available at https://github.com/ohlerlab/RiboseQC and submitted to Bioconductor.

Contact uwe.ohler{at}mdc-berlin.de

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted April 08, 2019.
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Ribo-seQC: comprehensive analysis of cytoplasmic and organellar ribosome profiling data
Lorenzo Calviello, Dominique Sydow, Dermot Harnett, Uwe Ohler
bioRxiv 601468; doi: https://doi.org/10.1101/601468
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Ribo-seQC: comprehensive analysis of cytoplasmic and organellar ribosome profiling data
Lorenzo Calviello, Dominique Sydow, Dermot Harnett, Uwe Ohler
bioRxiv 601468; doi: https://doi.org/10.1101/601468

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