Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Complex responses to inflammatory oxidants by the probiotic bacterium Lactobacillus reuteri

Poulami Basu Thakur, Abagail R. Long, Benjamin J. Nelson, Ranjit Kumar, Alexander F. Rosenberg, View ORCID ProfileMichael J. Gray
doi: https://doi.org/10.1101/605881
Poulami Basu Thakur
aDepartment of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abagail R. Long
aDepartment of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin J. Nelson
bAuburn University, Auburn, AL 36849
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ranjit Kumar
cCenter for Clinical and Translational Sciences, University of Alabama at Birmingham, Birmingham, AL 35294
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alexander F. Rosenberg
aDepartment of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294
dInformatics Institute, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael J. Gray
aDepartment of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael J. Gray
  • For correspondence: mjgray@uab.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Inflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes to dysbiotic changes in the gut microbiome, including increased populations of pro-inflammatory enterobacteria (Escherichia coli and related species) and decreased levels of health-associated anaerobic Firmicutes and Bacteroidetes. The pathways for H2O2 and HOCl resistance in E. coli have been well-studied, but little is known about how commensal and probiotic bacteria respond to inflammatory oxidants. In this work, we have characterized the transcriptomic response of the anti-inflammatory, gut-colonizing Gram-positive probiotic Lactobacillus reuteri to both H2O2 and HOCl. L. reuteri mounts distinct responses to each of these stressors, and both gene expression and survival were strongly affected by the presence or absence of oxygen. Oxidative stress response in L. reuteri required several factors not found in enterobacteria, including the small heat shock protein Lo18, polyphosphate kinase 2, and RsiR, an L. reuteri-specific regulator of anti-inflammatory mechanisms. These results raise the intriguing possibility of developing treatments for inflammatory gut diseases that could sensitize pro-inflammatory enterobacteria to killing by the immune system while sparing anti-inflammatory, health-associated species.

IMPORTANCE It is becoming increasingly clear that effective treatment of inflammatory gut diseases will require modulation of the gut microbiota. Preventing pro-inflammatory bacteria from blooming while also preserving anti-inflammatory and commensal species is a considerable challenge, but our results suggest that it may be possible to take advantage of differences in the way different species of gut bacteria resist inflammatory oxidants to accomplish this goal.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 11, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Complex responses to inflammatory oxidants by the probiotic bacterium Lactobacillus reuteri
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Complex responses to inflammatory oxidants by the probiotic bacterium Lactobacillus reuteri
Poulami Basu Thakur, Abagail R. Long, Benjamin J. Nelson, Ranjit Kumar, Alexander F. Rosenberg, Michael J. Gray
bioRxiv 605881; doi: https://doi.org/10.1101/605881
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Complex responses to inflammatory oxidants by the probiotic bacterium Lactobacillus reuteri
Poulami Basu Thakur, Abagail R. Long, Benjamin J. Nelson, Ranjit Kumar, Alexander F. Rosenberg, Michael J. Gray
bioRxiv 605881; doi: https://doi.org/10.1101/605881

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4091)
  • Biochemistry (8772)
  • Bioengineering (6487)
  • Bioinformatics (23356)
  • Biophysics (11756)
  • Cancer Biology (9154)
  • Cell Biology (13257)
  • Clinical Trials (138)
  • Developmental Biology (7418)
  • Ecology (11376)
  • Epidemiology (2066)
  • Evolutionary Biology (15095)
  • Genetics (10403)
  • Genomics (14014)
  • Immunology (9126)
  • Microbiology (22070)
  • Molecular Biology (8783)
  • Neuroscience (47395)
  • Paleontology (350)
  • Pathology (1421)
  • Pharmacology and Toxicology (2482)
  • Physiology (3705)
  • Plant Biology (8054)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2211)
  • Systems Biology (6017)
  • Zoology (1250)