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A 3D molecular atlas of the chick embryonic heart

Claire Anderson, Bill Hill, Hui-Chun Lu, Adam Moverley, Youwen Yang, Nidia M.M. Oliveira, Richard A. Baldock, View ORCID ProfileClaudio D. Stern
doi: https://doi.org/10.1101/609032
Claire Anderson
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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Bill Hill
MRC Human Genetics Unit, IGMM, University of Edinburgh, EH4 2XU, UK
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Hui-Chun Lu
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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Adam Moverley
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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Youwen Yang
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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Nidia M.M. Oliveira
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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Richard A. Baldock
MRC Human Genetics Unit, IGMM, University of Edinburgh, EH4 2XU, UK
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Claudio D. Stern
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK
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  • ORCID record for Claudio D. Stern
  • For correspondence: c.stern@ucl.ac.uk
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Abstract

We present a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq of 13 micro-dissected regions reveals regionalised expression of 15,570 genes. Of these, 132 were studied by in situ hybridisation and a subset (38 genes) was mapped by Optical Projection Tomography or serial sectioning to build a detailed 3-dimensional atlas of expression. We display this with a novel interactive 3-D viewer and as stacks of sections, revealing the boundaries of expression domains and regions of overlap. Analysis of the expression domains also defines some sub-regions distinct from those normally recognised by anatomical criteria at this stage of development, such as a previously undescribed subdivision of the atria into two orthogonal sets of domains (dorsoventral and left-right). We also include a detailed comparison of expression in the chick with the mouse and other species.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted April 16, 2019.
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A 3D molecular atlas of the chick embryonic heart
Claire Anderson, Bill Hill, Hui-Chun Lu, Adam Moverley, Youwen Yang, Nidia M.M. Oliveira, Richard A. Baldock, Claudio D. Stern
bioRxiv 609032; doi: https://doi.org/10.1101/609032
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A 3D molecular atlas of the chick embryonic heart
Claire Anderson, Bill Hill, Hui-Chun Lu, Adam Moverley, Youwen Yang, Nidia M.M. Oliveira, Richard A. Baldock, Claudio D. Stern
bioRxiv 609032; doi: https://doi.org/10.1101/609032

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