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Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation

Artur Matysik, Kimberly A. Kline
doi: https://doi.org/10.1101/609065
Artur Matysik
1Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore
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Kimberly A. Kline
1Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore
2School of Biological Sciences, Nanyang Technological University, Singapore
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ABSTRACT

Biofilms play an important role in the pathogenesis of Group A Streptococcus (GAS), a gram-positive pathogen responsible for a wide range infections and significant public health impact. Although most GAS serotypes are able to form biofilms, there is large heterogeneity between individual strains in biofilm formation, as measured by standard crystal violet assays. It is generally accepted that biofilm formation includes initial adhesion of bacterial cells to a surface, followed by microcolony formation, biofilm maturation, and extensive production of extracellular matrix that links together proliferating cells and provides a scaffold for the three-dimensional biofilm structure. However, our studies show that for GAS strain JS95, microcolony formation is not an essential step in static biofilm formation, and instead, biofilm can be effectively formed from slow-growing or non-replicating late exponential or early stationary planktonic cells, via sedimentation and fixation of GAS chains into biofilms. In addition, we show that the GAS capsule specifically contributes to the alternative, sedimentation-initiated biofilms. Microcolony-independent, sedimentation biofilms are similar in morphology and 3-D structure to biofilms initiated by actively dividing planktonic bacteria. We conclude that GAS can form biofilms by an alternate, non-canonical mechanism that does not require transition from microcolony formation to biofilm maturation, and which may be obscured by biofilm phenotypes that arise via the classical biofilm maturation processes.

IMPORTANCE The static biofilm assay is a common tool for easy biomass quantification of biofilm forming bacteria. However, S. pyogenes biofilm formation as measured by the static assay is strain dependent and yields heterogeneous results for different strains of the same serotype. In this study, we show that two independent mechanisms, for which the protective capsule contributes opposing functions, may contribute to static biofilm formation. We propose that separation of these mechanisms for biofilm formation might uncover previously unappreciated biofilm phenotypes that may otherwise be masked in the classic static assay.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 15, 2019.
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Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
Artur Matysik, Kimberly A. Kline
bioRxiv 609065; doi: https://doi.org/10.1101/609065
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Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
Artur Matysik, Kimberly A. Kline
bioRxiv 609065; doi: https://doi.org/10.1101/609065

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