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Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants

Cecilia Cheval, Matthew Johnston, Sebastian Samwald, Xiaokun Liu, Annalisa Bellandi, Andrew Breakspear, Yasuhiro Kadota, Cyril Zipfel, Christine Faulkner
doi: https://doi.org/10.1101/611582
Cecilia Cheval
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Matthew Johnston
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Sebastian Samwald
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Xiaokun Liu
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Annalisa Bellandi
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Andrew Breakspear
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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Yasuhiro Kadota
2The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
4RIKEN Centre for Sustainable Resource Science, Tsurumi‐ku, Yokohama, 230‐0045, Japan
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Cyril Zipfel
2The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
3Institute of Plant and Microbial Biology, and Zürich-Basel Plant Science Centre, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland
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Christine Faulkner
1John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK
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  • For correspondence: christine.faulkner@jic.ac.uk
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Abstract

The plasma membrane (PM) that lines plasmodesmata has a distinct protein and lipid composition, underpinning specific regulation of these connections between cells. The plasmodesmal PM can integrate extracellular signals differently from the cellular PM, but it is not known how this specificity is established or how a single stimulus can trigger independent signalling cascades in neighbouring membrane domains. Here we have used the fungal elicitor chitin to investigate signal integration and responses at the plasmodesmal PM. We found that the plasmodesmal PM employs a receptor complex composed of the LysM receptors LYM2 and LYK4 which respectively change their location and interactions in response to chitin. Downstream, signalling is transmitted via a specific phosphorylation signature of an NADPH oxidase and localised callose synthesis that causes plasmodesmata closure. This demonstrates the plasmodesmal PM deploys both plasmodesmata-specific components and differential activation of PM-common components to independently integrate an immune signal.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted April 17, 2019.
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Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants
Cecilia Cheval, Matthew Johnston, Sebastian Samwald, Xiaokun Liu, Annalisa Bellandi, Andrew Breakspear, Yasuhiro Kadota, Cyril Zipfel, Christine Faulkner
bioRxiv 611582; doi: https://doi.org/10.1101/611582
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Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants
Cecilia Cheval, Matthew Johnston, Sebastian Samwald, Xiaokun Liu, Annalisa Bellandi, Andrew Breakspear, Yasuhiro Kadota, Cyril Zipfel, Christine Faulkner
bioRxiv 611582; doi: https://doi.org/10.1101/611582

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