Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Scalable, FACS-Free Genome-Wide Phenotypic Screening

View ORCID ProfileBarbara Mair, Peter M. Aldridge, Randy S. Atwal, Sanna N. Masud, Meng Zhang, David Philpott, Amy H.Y. Tong, Edward H. Sargent, View ORCID ProfileStéphane Angers, Jason Moffat, Shana O. Kelley
doi: https://doi.org/10.1101/612887
Barbara Mair
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Barbara Mair
Peter M. Aldridge
Leslie Dan Faculty of Pharmacy, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Randy S. Atwal
Leslie Dan Faculty of Pharmacy, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sanna N. Masud
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, ON, Canada,Department of Molecular Genetics, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meng Zhang
Leslie Dan Faculty of Pharmacy, University of Toronto, ON, Canada,Department of Biochemistry, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David Philpott
Department of Electrical & Computer Engineering, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amy H.Y. Tong
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edward H. Sargent
Department of Electrical & Computer Engineering, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stéphane Angers
Leslie Dan Faculty of Pharmacy, University of Toronto, ON, Canada,Department of Biochemistry, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stéphane Angers
Jason Moffat
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, ON, Canada,Department of Molecular Genetics, University of Toronto, ON, Canada,Canadian Institute for Advanced Research, Toronto, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: shana.kelley@utoronto.ca j.moffat@utoronto.ca
Shana O. Kelley
Leslie Dan Faculty of Pharmacy, University of Toronto, ON, Canada,Department of Biochemistry, University of Toronto, ON, Canada,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: shana.kelley@utoronto.ca j.moffat@utoronto.ca
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Genome-scale functional genetic screens can identify key regulators of a phenotype of interest, such as determinants of protein expression or modification. Here, we present a rapid, high-throughput approach to phenotypic CRISPR-Cas9 screening. To study factors that modulate the display of CD47 on the cell surface, we processed an entire genome-wide screen containing more than 108 cells in under one hour and maintained high levels of cell viability using a highly scalable cell sorting technology. We robustly identified modulators of CD47 function including QPCTL, an enzyme required for formation of the pyroglutamyl modification at the N-terminus of this protein.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 19, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Scalable, FACS-Free Genome-Wide Phenotypic Screening
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Scalable, FACS-Free Genome-Wide Phenotypic Screening
Barbara Mair, Peter M. Aldridge, Randy S. Atwal, Sanna N. Masud, Meng Zhang, David Philpott, Amy H.Y. Tong, Edward H. Sargent, Stéphane Angers, Jason Moffat, Shana O. Kelley
bioRxiv 612887; doi: https://doi.org/10.1101/612887
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Scalable, FACS-Free Genome-Wide Phenotypic Screening
Barbara Mair, Peter M. Aldridge, Randy S. Atwal, Sanna N. Masud, Meng Zhang, David Philpott, Amy H.Y. Tong, Edward H. Sargent, Stéphane Angers, Jason Moffat, Shana O. Kelley
bioRxiv 612887; doi: https://doi.org/10.1101/612887

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (1524)
  • Biochemistry (2479)
  • Bioengineering (1731)
  • Bioinformatics (9670)
  • Biophysics (3896)
  • Cancer Biology (2968)
  • Cell Biology (4189)
  • Clinical Trials (135)
  • Developmental Biology (2624)
  • Ecology (4098)
  • Epidemiology (2031)
  • Evolutionary Biology (6894)
  • Genetics (5204)
  • Genomics (6496)
  • Immunology (2183)
  • Microbiology (6937)
  • Molecular Biology (2751)
  • Neuroscience (17261)
  • Paleontology (126)
  • Pathology (425)
  • Pharmacology and Toxicology (705)
  • Physiology (1056)
  • Plant Biology (2488)
  • Scientific Communication and Education (643)
  • Synthetic Biology (831)
  • Systems Biology (2687)
  • Zoology (429)