ABSTRACT
VDJPuzzle and VDJView form a tool for simultaneous analysis and visualisation of gene expression and immune receptors from the scRNAseq data of both T and B cells, that allows accurate detection of secreted/membrane bound B cell receptors. Using VDJPuzzle on scRNAseq data from breast cancer and metastatic lymph-nodes, we identified T and B cell subsets with distinct patterns of hypermutation, isotype, and gene signature associated with immune cell differentiation and activation.
Footnotes
The tools are available on BitBucket: https://bitbucket.org/kirbyvisp/vdjpuzzle https://bitbucket.org/kirbyvisp/vdjview
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.