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A Method for Cost-Effective and Rapid Characterization of Engineered T7-based Transcription Factors by Cell-Free Protein Synthesis Reveals Insights into the Regulation of T7 RNA Polymerase-Driven Expression

John B. McManus, Richard M. Murray, Peter A. Emanuel, Matthew W. Lux
doi: https://doi.org/10.1101/614545
John B. McManus
aUS Army Combat Capabilities Development Command, Army Research Laboratory, 2800 Powder Mill Rd, Adelphi, MD 20783
bCalifornia Institute of Technology, Biology and Biological Engineering, 1200 East California Blvd, Pasadena, CA 91125
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Richard M. Murray
bCalifornia Institute of Technology, Biology and Biological Engineering, 1200 East California Blvd, Pasadena, CA 91125
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Peter A. Emanuel
CUS Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, APG, MD 21010
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Matthew W. Lux
CUS Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, APG, MD 21010
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  • For correspondence: matthew.w.lux.civ@mail.mil
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Posted April 20, 2019.
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A Method for Cost-Effective and Rapid Characterization of Engineered T7-based Transcription Factors by Cell-Free Protein Synthesis Reveals Insights into the Regulation of T7 RNA Polymerase-Driven Expression
John B. McManus, Richard M. Murray, Peter A. Emanuel, Matthew W. Lux
bioRxiv 614545; doi: https://doi.org/10.1101/614545
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A Method for Cost-Effective and Rapid Characterization of Engineered T7-based Transcription Factors by Cell-Free Protein Synthesis Reveals Insights into the Regulation of T7 RNA Polymerase-Driven Expression
John B. McManus, Richard M. Murray, Peter A. Emanuel, Matthew W. Lux
bioRxiv 614545; doi: https://doi.org/10.1101/614545

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