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Tracking the cells of tumor origin in breast organoids by light sheet microscopy

A Alladin, L Chaible, S Reither, M Löschinger, M Wachsmuth, JK Hériché, C Tischer, M Jechlinger
doi: https://doi.org/10.1101/617837
A Alladin
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany
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L Chaible
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany
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S Reither
Advanced Light Microscopy Facility, EMBL, Heidelberg, Germany
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M Löschinger
Luxendo Light-Sheet, Bruker Corporation, Heidelberg, Germany
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M Wachsmuth
Luxendo Light-Sheet, Bruker Corporation, Heidelberg, Germany
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JK Hériché
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany
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C Tischer
Centre for Bioimage Analysis, EMBL, Heidelberg, Germany
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M Jechlinger
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany
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  • For correspondence: martin.jechlinger@embl.de
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Abstract

How tumors arise from individual transformed cells within an intact epithelium is a central, yet unanswered question. Here, we developed a new methodology that combines breast tissue organoids, where oncogenes can be switched on in single cells, with light-sheet imaging that allows us to track cell fates using a big-image-data analysis workflow. The power of this integrated approach is illustrated by our finding that small local groups of transformed cells form tumors while isolated transformed cells do not.

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Posted April 24, 2019.
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Tracking the cells of tumor origin in breast organoids by light sheet microscopy
A Alladin, L Chaible, S Reither, M Löschinger, M Wachsmuth, JK Hériché, C Tischer, M Jechlinger
bioRxiv 617837; doi: https://doi.org/10.1101/617837
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Tracking the cells of tumor origin in breast organoids by light sheet microscopy
A Alladin, L Chaible, S Reither, M Löschinger, M Wachsmuth, JK Hériché, C Tischer, M Jechlinger
bioRxiv 617837; doi: https://doi.org/10.1101/617837

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