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Throughput and Resolution with a Next Generation Direct Electron Detector

View ORCID ProfileJoshua H. Mendez, View ORCID ProfileAtousa Mehrani, View ORCID ProfilePeter Randolph, View ORCID ProfileScott Stagg
doi: https://doi.org/10.1101/620617
Joshua H. Mendez
1Department of Physics, 77 Chieftan Way, Tallahassee, FL 32306
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Atousa Mehrani
2Department of Chemistry and Biochemistry, 95 Chieftain Way, Florida State University, Tallahassee, FL 32306
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Peter Randolph
3Institute of Molecular Biophysics, 91 Chieftain Way, Florida State University, Tallahassee, FL 32306
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Scott Stagg
2Department of Chemistry and Biochemistry, 95 Chieftain Way, Florida State University, Tallahassee, FL 32306
3Institute of Molecular Biophysics, 91 Chieftain Way, Florida State University, Tallahassee, FL 32306
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  • For correspondence: sstagg@fsu.edu
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Abstract

Direct electron detectors (DEDs) have revolutionized cryo-electron microscopy by facilitating correction of beam-induced motion and radiation damage and by providing high-resolution image capture. A new generation of DEDs has been developed by Direct Electron, the DE64, that has good performance in both integrating and counting modes. Integrating mode is superior in throughput while counting mode is superior in image quality. We show that despite being ~10x slower in throughput, counting mode is superior in terms of reconstruction resolution per unit time of data collection.

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Posted May 03, 2019.
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Throughput and Resolution with a Next Generation Direct Electron Detector
Joshua H. Mendez, Atousa Mehrani, Peter Randolph, Scott Stagg
bioRxiv 620617; doi: https://doi.org/10.1101/620617
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Throughput and Resolution with a Next Generation Direct Electron Detector
Joshua H. Mendez, Atousa Mehrani, Peter Randolph, Scott Stagg
bioRxiv 620617; doi: https://doi.org/10.1101/620617

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