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Total RNA-sequencing reveals multi-level microbial community changes and functional responses to wood ash application in agricultural and forest soil

Toke Bang-Andreasen, Muhammad Zohaib Anwar, Anders Lanzén, Rasmus Kjøller, Regin Rønn, Flemming Ekelund, Carsten Suhr Jacobsen
doi: https://doi.org/10.1101/621557
Toke Bang-Andreasen
1Department of Environmental Science, Aarhus University, RISØ campus, Roskilde, Denmark
2Department of Biology, University of Copenhagen, Copenhagen, Denmark
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Muhammad Zohaib Anwar
1Department of Environmental Science, Aarhus University, RISØ campus, Roskilde, Denmark
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Anders Lanzén
3Department of Conservation of Natural Resources, NEIKER-Tecnalia, Bizkaia Technology Park, Derio, Spain
4AZTI-Tecnalia, Herrera Kaia, Pasaia, Spain
5IKERBASQUE, Basque Foundation for Science, Bilbao, Spain
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Rasmus Kjøller
2Department of Biology, University of Copenhagen, Copenhagen, Denmark
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Regin Rønn
2Department of Biology, University of Copenhagen, Copenhagen, Denmark
6Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China
7Arctic Station, University of Copenhagen, Qeqertarsuaq, Greenland
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Flemming Ekelund
2Department of Biology, University of Copenhagen, Copenhagen, Denmark
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  • For correspondence: fekelund@bio.ku.dk
Carsten Suhr Jacobsen
1Department of Environmental Science, Aarhus University, RISØ campus, Roskilde, Denmark
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Abstract

Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied Total RNA-sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, Total RNA-sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.

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Posted April 30, 2019.
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Total RNA-sequencing reveals multi-level microbial community changes and functional responses to wood ash application in agricultural and forest soil
Toke Bang-Andreasen, Muhammad Zohaib Anwar, Anders Lanzén, Rasmus Kjøller, Regin Rønn, Flemming Ekelund, Carsten Suhr Jacobsen
bioRxiv 621557; doi: https://doi.org/10.1101/621557
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Total RNA-sequencing reveals multi-level microbial community changes and functional responses to wood ash application in agricultural and forest soil
Toke Bang-Andreasen, Muhammad Zohaib Anwar, Anders Lanzén, Rasmus Kjøller, Regin Rønn, Flemming Ekelund, Carsten Suhr Jacobsen
bioRxiv 621557; doi: https://doi.org/10.1101/621557

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