Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Generation of transgenic quails by in vivo transfection of primordial germ cells

Olivier Serralbo, Nadège Véron, Caitlin Cooper, Marie-Julie Dejardin, Timothy Doran, Christophe Marcelle
doi: https://doi.org/10.1101/625665
Olivier Serralbo
EMBL Australia; Australian Regenerative Medicine Institute (ARMI), Monash University, Building 75, Clayton, Victoria 3800, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: olivier.serralbo@monash.edu christophe.marcelle@monash.edu
Nadège Véron
EMBL Australia; Australian Regenerative Medicine Institute (ARMI), Monash University, Building 75, Clayton, Victoria 3800, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caitlin Cooper
CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong Vic, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marie-Julie Dejardin
Institut NeuroMyoGène (INMG), University Claude Bernard Lyon1, CNRS UMR 5310, INSERM U1217, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Timothy Doran
CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong Vic, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christophe Marcelle
EMBL Australia; Australian Regenerative Medicine Institute (ARMI), Monash University, Building 75, Clayton, Victoria 3800, AustraliaInstitut NeuroMyoGène (INMG), University Claude Bernard Lyon1, CNRS UMR 5310, INSERM U1217, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: olivier.serralbo@monash.edu christophe.marcelle@monash.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

During development, dynamic changes in tissue shapes known as morphogenesis result from the exquisite orchestration of signalling pathways, cell–cell interactions, cell divisions, and coordinated movements. How cells within embryos adopt a vast array of cell fates and tissue shapes in such an ever-changing environment has fascinated scientists for generations, yet the ability to observe and characterize those rapid changes has proven technically challenging in higher vertebrates. The japanese quail (Coturnix coturnix japonica) is an attractive model where basic rules driving morphogenesis in amniotes can be deciphered using genetic approaches. Similar to the more popular chicken model, the quail embryo is easily accessible to a wide range of manipulations and live imaging. A decisive asset of quail over chicken is a much shorter life cycle, which makes its use as genetic model for basic research extremely appealing. To date, all existing transgenic quail lines were generated using replication-deficient lentiviruses, but diverse limitations of this approach have hindered the widespread expansion of such technology. Here, we successfully used a plasmid-based in vivo transfection of quail primordial germ cells (PGCs) to generate a number of transgenic quail lines over a short period of time. The plasmid-based approach is simple, efficient and it allows using the infinite variety of genome engineering approaches developed in other models, such as strategies to facilitate transgenic bird selection, shown here. This major technological development completes the vast panel of techniques applicable to the avian model, making it one of the most versatile experimental systems available.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted May 02, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Generation of transgenic quails by in vivo transfection of primordial germ cells
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Generation of transgenic quails by in vivo transfection of primordial germ cells
Olivier Serralbo, Nadège Véron, Caitlin Cooper, Marie-Julie Dejardin, Timothy Doran, Christophe Marcelle
bioRxiv 625665; doi: https://doi.org/10.1101/625665
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Generation of transgenic quails by in vivo transfection of primordial germ cells
Olivier Serralbo, Nadège Véron, Caitlin Cooper, Marie-Julie Dejardin, Timothy Doran, Christophe Marcelle
bioRxiv 625665; doi: https://doi.org/10.1101/625665

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1527)
  • Biochemistry (2480)
  • Bioengineering (1739)
  • Bioinformatics (9683)
  • Biophysics (3903)
  • Cancer Biology (2972)
  • Cell Biology (4195)
  • Clinical Trials (135)
  • Developmental Biology (2627)
  • Ecology (4102)
  • Epidemiology (2031)
  • Evolutionary Biology (6898)
  • Genetics (5206)
  • Genomics (6501)
  • Immunology (2184)
  • Microbiology (6945)
  • Molecular Biology (2753)
  • Neuroscience (17282)
  • Paleontology (126)
  • Pathology (427)
  • Pharmacology and Toxicology (706)
  • Physiology (1057)
  • Plant Biology (2489)
  • Scientific Communication and Education (643)
  • Synthetic Biology (831)
  • Systems Biology (2690)
  • Zoology (430)