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Taxonomic resolution of the ribosomal RNA operon in bacteria: Implications for its use with long read sequencing

View ORCID ProfileLeonardo de Oliveira Martins, View ORCID ProfileAndrew J. Page, Ian G. Charles
doi: https://doi.org/10.1101/626093
Leonardo de Oliveira Martins
Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
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  • ORCID record for Leonardo de Oliveira Martins
  • For correspondence: Leonardo.de-Oliveira-Martins@quadram.ac.uk
Andrew J. Page
Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
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Ian G. Charles
Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
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Abstract

Long-read sequencing technologies enable capture of the full-length of ribosomal RNA operons in a single read. Bacterial cells usually have multiple copies of this ribosomal operon; sequence variation within a species of bacterium can exceed variation between species. For uncultured organisms this may affect the overall taxonomic resolution, to genus level, of the full-length ribosomal operon.

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  • https://github.com/quadram-institute-bioscience/70S-resolution

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 02, 2019.
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Taxonomic resolution of the ribosomal RNA operon in bacteria: Implications for its use with long read sequencing
Leonardo de Oliveira Martins, Andrew J. Page, Ian G. Charles
bioRxiv 626093; doi: https://doi.org/10.1101/626093
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Taxonomic resolution of the ribosomal RNA operon in bacteria: Implications for its use with long read sequencing
Leonardo de Oliveira Martins, Andrew J. Page, Ian G. Charles
bioRxiv 626093; doi: https://doi.org/10.1101/626093

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