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Simultaneous regulation of cytokinetic furrow and nucleus positions by cortical tension contributes to proper DNA segregation during late mitosis

Anne Pacquelet, Matthieu Jousseaume, View ORCID ProfileJocelyn Etienne, View ORCID ProfileGrégoire Michaux
doi: https://doi.org/10.1101/626903
Anne Pacquelet
1Univ Rennes, CNRS, IGDR (Institut de Génétique et de Développement de Rennes), UMR 6290, F-35000 Rennes, France
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  • For correspondence: anne.pacquelet@univ-rennes1.fr
Matthieu Jousseaume
1Univ Rennes, CNRS, IGDR (Institut de Génétique et de Développement de Rennes), UMR 6290, F-35000 Rennes, France
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Jocelyn Etienne
2Univ Grenoble Alpes, CNRS, LIPhy, 38000 Grenoble, France
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Grégoire Michaux
1Univ Rennes, CNRS, IGDR (Institut de Génétique et de Développement de Rennes), UMR 6290, F-35000 Rennes, France
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Abstract

Coordinating mitotic spindle and cytokinetic furrow positioning is essential to ensure proper DNA segregation. Here we present a novel mechanism, which corrects DNA segregation defects due to cytokinetic furrow mispositioning. We show that DNA segregation defects following the abnormal displacement of the cytokinetic furrow towards the anterior side of C. elegans one-cell embryos are unexpectedly corrected at the end of cytokinesis. This correction relies on the concomitant displacement of the furrow and of the anterior nucleus towards the posterior and anterior poles, respectively. It also coincides with cortical blebbing and an anteriorly directed flow of cytoplasmic particles. While microtubules contribute to nuclear displacement, relaxation of an excessive tension at the anterior cortex plays a central role in the correction process and simultaneously regulates cytoplasmic flow as well as nuclear and furrow displacements. This work thus reveals the existence of a so far uncharacterized correction mechanism, which is critical to correct DNA segregation defects due to cytokinetic furrow mispositioning.

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Posted May 15, 2019.
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Simultaneous regulation of cytokinetic furrow and nucleus positions by cortical tension contributes to proper DNA segregation during late mitosis
Anne Pacquelet, Matthieu Jousseaume, Jocelyn Etienne, Grégoire Michaux
bioRxiv 626903; doi: https://doi.org/10.1101/626903
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Simultaneous regulation of cytokinetic furrow and nucleus positions by cortical tension contributes to proper DNA segregation during late mitosis
Anne Pacquelet, Matthieu Jousseaume, Jocelyn Etienne, Grégoire Michaux
bioRxiv 626903; doi: https://doi.org/10.1101/626903

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