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Computational and experimental optimization of T cell activation

View ORCID ProfileBulent Arman Aksoy, View ORCID ProfileEric Czech, View ORCID ProfileChrystal Paulos, View ORCID ProfileJeff Hammerbacher
doi: https://doi.org/10.1101/629857
Bulent Arman Aksoy
*Microbiology and Immunology Department at the Medical University of South Carolina
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  • For correspondence: arman@hammerlab.org
Eric Czech
*Microbiology and Immunology Department at the Medical University of South Carolina
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Chrystal Paulos
*Microbiology and Immunology Department at the Medical University of South Carolina
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Jeff Hammerbacher
*Microbiology and Immunology Department at the Medical University of South Carolina
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Abstract

Bead-based activation is widely-used for ex vivo expansion of T cells for either research or clinical purposes. Despite its wide use, culture conditions that can potentially affect the efficiency of bead-based T cell activation has not been extensively documented. With the help of computationally-driven experimental investigations of basic culturing factors, we found that culture density, bead-to-cell ratio, and debeading time can have a major impact on the efficiency of bead-based T cell activation for short-term cultures. Furthermore, discrepancies across expected and observed activation efficiencies helped discover interesting artifacts of bead-based T cell activation.

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Human primary T cells were imaged together with activation beads at 20X magnification after three hours of culturing at varying confluencies and bead-to-cell ratios.

Footnotes

  • https://github.com/hammerlab/t-cell-activation

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 07, 2019.
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Computational and experimental optimization of T cell activation
Bulent Arman Aksoy, Eric Czech, Chrystal Paulos, Jeff Hammerbacher
bioRxiv 629857; doi: https://doi.org/10.1101/629857
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Computational and experimental optimization of T cell activation
Bulent Arman Aksoy, Eric Czech, Chrystal Paulos, Jeff Hammerbacher
bioRxiv 629857; doi: https://doi.org/10.1101/629857

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