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COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast

View ORCID ProfileYoulian Goulev, Audrey Matifas, Vincent Heyer, View ORCID ProfileBernardo Reina-San-Martin, View ORCID ProfileGilles Charvin
doi: https://doi.org/10.1101/630277
Youlian Goulev
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • For correspondence: youlian.goulev@gmail.com charvin@igbmc.fr
Audrey Matifas
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Vincent Heyer
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Bernardo Reina-San-Martin
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Bernardo Reina-San-Martin
Gilles Charvin
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Gilles Charvin
  • For correspondence: youlian.goulev@gmail.com charvin@igbmc.fr
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Abstract

A large number of genetic studies in yeast rely on the use of expression vectors. To facilitate the experimental approach of these studies, several collections of expression vectors have been generated (YXplac, pRS series, etc.). Subsequently, these collections have been expanded by adding more diversity to many of the plasmid features, including new selection markers and new promoter sequences. However, the ever growing number of plasmid feature makes it unrealistic for research labs to maintain an up-to-date collection of plasmids.. Here, we developed the COSPLAY toolbox: a Golden Gate approach based on the scheme of a simple modular plasmid that recapitulates and completes all the properties of the pRS plasmids. The COSPLAY toolbox contains a basal collection of individual functional modules. Moreover we standardized a simple and rapid, software-assisted protocol which facilitates the addition of new personalized modules. Finally, our toolbox includes the possibility to select a genomic target location and to perform a single copy integration of the expression vector.

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  • https://github.com/gcharvin/cosplay

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 07, 2019.
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COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast
Youlian Goulev, Audrey Matifas, Vincent Heyer, Bernardo Reina-San-Martin, Gilles Charvin
bioRxiv 630277; doi: https://doi.org/10.1101/630277
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COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast
Youlian Goulev, Audrey Matifas, Vincent Heyer, Bernardo Reina-San-Martin, Gilles Charvin
bioRxiv 630277; doi: https://doi.org/10.1101/630277

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