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Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis

Jo-Ann Chuah, Masaki Odahara, Yutaka Kodama, Takaaki Miyamoto, Kousuke Tsuchiya, Yoko Motoda, Takanori Kigawa, Keiji Numata
doi: https://doi.org/10.1101/630301
Jo-Ann Chuah
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
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Masaki Odahara
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
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Yutaka Kodama
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
2Center for Bioscience Research and Education, Utsunomiya University, Tochigi 321-8505, Japan.
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Takaaki Miyamoto
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
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Kousuke Tsuchiya
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
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Yoko Motoda
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
3Laboratory for Cellular Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan.
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Takanori Kigawa
3Laboratory for Cellular Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, Japan.
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Keiji Numata
1Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama 351-0198, Japan.
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  • For correspondence: keiji.numata@riken.jp
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Summary

Efficient intracellular delivery of biomolecules is important for many different biological and biotechnological applications in living organisms, and is a prerequisite for certain types of fundamental and applied research. One major challenge is the delivery of unmodified, functional cargoes in a simple, time-efficient, and high-throughput manner. Herein, we present an efficient strategy that uses fusion peptides containing cell penetrating peptide, endosomal escape domain, and a sarcosine linker to introduce biomolecules, namely fluorescent protein and dextran, via macropinocytosis into the cells of various land plants and microalgae. Our peptide-mediated delivery system allows for high-throughput delivery of functional biomolecules within a few minutes to a few hours as well as open new possibilities for biology and biotechnology using difficult-to-transfect cell types.

Footnotes

  • Conflict of interest statement: No conflicts declared.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 07, 2019.
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Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis
Jo-Ann Chuah, Masaki Odahara, Yutaka Kodama, Takaaki Miyamoto, Kousuke Tsuchiya, Yoko Motoda, Takanori Kigawa, Keiji Numata
bioRxiv 630301; doi: https://doi.org/10.1101/630301
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Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis
Jo-Ann Chuah, Masaki Odahara, Yutaka Kodama, Takaaki Miyamoto, Kousuke Tsuchiya, Yoko Motoda, Takanori Kigawa, Keiji Numata
bioRxiv 630301; doi: https://doi.org/10.1101/630301

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