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Protein structure prediction and design in a biologically-realistic implicit membrane

View ORCID ProfileRebecca F. Alford, View ORCID ProfilePatrick J. Fleming, Karen G. Fleming, View ORCID ProfileJeffrey J. Gray
doi: https://doi.org/10.1101/630715
Rebecca F. Alford
aDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218
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Patrick J. Fleming
bT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218
cProgram in Molecular Biophysics, Johns Hopkins University, Baltimore, MD 21218
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Karen G. Fleming
bT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218
cProgram in Molecular Biophysics, Johns Hopkins University, Baltimore, MD 21218
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Jeffrey J. Gray
aDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218
cProgram in Molecular Biophysics, Johns Hopkins University, Baltimore, MD 21218
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  • For correspondence: jgray@jhu.edu
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ABSTRACT

Protein design is a powerful tool for elucidating mechanisms of function and engineering new therapeutics and nanotechnologies. While soluble protein design has advanced, membrane protein design remains challenging due to difficulties in modeling the lipid bilayer. In this work, we developed an implicit approach that captures the anisotropic structure, shape of water-filled pores, and nanoscale dimensions of membranes with different lipid compositions. The model improves performance in computational bench-marks against experimental targets including prediction of protein orientations in the bilayer, ΔΔG calculations, native structure dis-crimination, and native sequence recovery. When applied to de novo protein design, this approach designs sequences with an amino acid distribution near the native amino acid distribution in membrane proteins, overcoming a critical flaw in previous membrane models that were prone to generating leucine-rich designs. Further, the proteins designed in the new membrane model exhibit native-like features including interfacial aromatic side chains, hydrophobic lengths compatible with bilayer thickness, and polar pores. Our method advances high-resolution membrane protein structure prediction and design toward tackling key biological questions and engineering challenges.

Significance Statement Membrane proteins participate in many life processes including transport, signaling, and catalysis. They constitute over 30% of all proteins and are targets for over 60% of pharmaceuticals. Computational design tools for membrane proteins will transform the interrogation of basic science questions such as membrane protein thermodynamics and the pipeline for engineering new therapeutics and nanotechnologies. Existing tools are either too expensive to compute or rely on manual design strategies. In this work, we developed a fast and accurate method for membrane protein design. The tool is available to the public and will accelerate the experimental design pipeline for membrane proteins.

  • membrane proteins
  • lipid composition
  • energy function
  • structure prediction
  • protein design
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Posted May 08, 2019.
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Protein structure prediction and design in a biologically-realistic implicit membrane
Rebecca F. Alford, Patrick J. Fleming, Karen G. Fleming, Jeffrey J. Gray
bioRxiv 630715; doi: https://doi.org/10.1101/630715
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Protein structure prediction and design in a biologically-realistic implicit membrane
Rebecca F. Alford, Patrick J. Fleming, Karen G. Fleming, Jeffrey J. Gray
bioRxiv 630715; doi: https://doi.org/10.1101/630715

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