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A bidirectional network for appetite control in zebrafish

Caroline Lei Wee, Erin Yue Song, Robert Evan Johnson, Deepak Ailani, Owen Randlett, Jiyoon Kim, Maxim Nikitchenko, Armin Bahl, Chao-Tsung Yang, Misha Ahrens, Koichi Kawakami, Florian Engert, Samuel Kunes
doi: https://doi.org/10.1101/631341
Caroline Lei Wee
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
2Program in Neuroscience, Harvard University, Boston, MA, USA
5Institute of Molecular and Cell Biology, A*STAR, Singapore
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  • For correspondence: carolinewee@gmail.com
Erin Yue Song
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Robert Evan Johnson
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
2Program in Neuroscience, Harvard University, Boston, MA, USA
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Deepak Ailani
3Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Shizuoka, Japan
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Owen Randlett
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Jiyoon Kim
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Maxim Nikitchenko
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Armin Bahl
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Chao-Tsung Yang
4Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
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Misha Ahrens
4Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
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Koichi Kawakami
3Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Shizuoka, Japan
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Florian Engert
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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Samuel Kunes
1Department of Molecular and Cell Biology, Harvard University, Cambridge, MA, USA
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ABSTRACT

Medial and lateral hypothalamic loci are known to suppress and enhance appetite, respectively, but their interactions and dynamics have not yet been explored. Here we report that, in zebrafish, serotonergic neurons of the ventromedial caudal hypothalamus (cH) become increasingly active during food deprivation, whereas activity in the lateral hypothalamus (LH) is reduced. Exposure to food sensory and consummatory cues reverses the activity states of these two nuclei, reflecting an opposing internal hunger state induced by food. An intermediate activity state is restored as satiety approaches. The overall antagonistic relationship of cH and LH was confirmed by simultaneous calcium imaging, and a causal relationship was established by targeted stimulation and ablation of the cH. The collective data allows us to propose a model in which activities in anti-correlated hypothalamic nuclei direct distinct phases of hunger, and thus coordinate energy balance via mutually antagonistic control of distinct behavioral outputs.

Footnotes

  • Corrected typos within the text and minor figure mistakes (particularly Figure 2 - S4 and 5). We have also made some minor clarifications within figure legends. One author was added.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 19, 2019.
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A bidirectional network for appetite control in zebrafish
Caroline Lei Wee, Erin Yue Song, Robert Evan Johnson, Deepak Ailani, Owen Randlett, Jiyoon Kim, Maxim Nikitchenko, Armin Bahl, Chao-Tsung Yang, Misha Ahrens, Koichi Kawakami, Florian Engert, Samuel Kunes
bioRxiv 631341; doi: https://doi.org/10.1101/631341
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A bidirectional network for appetite control in zebrafish
Caroline Lei Wee, Erin Yue Song, Robert Evan Johnson, Deepak Ailani, Owen Randlett, Jiyoon Kim, Maxim Nikitchenko, Armin Bahl, Chao-Tsung Yang, Misha Ahrens, Koichi Kawakami, Florian Engert, Samuel Kunes
bioRxiv 631341; doi: https://doi.org/10.1101/631341

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