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Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings

View ORCID ProfileStéphane Vassilopoulos, Solène Gibaud, Angélique Jimenez, Ghislaine Caillol, View ORCID ProfileChristophe Leterrier
doi: https://doi.org/10.1101/636217
Stéphane Vassilopoulos
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, Paris, France
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  • ORCID record for Stéphane Vassilopoulos
  • For correspondence: s.vassilopoulos@institut-myologie.org christophe.leterrier@univ-amu.fr
Solène Gibaud
2Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France
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Angélique Jimenez
2Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France
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Ghislaine Caillol
2Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France
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Christophe Leterrier
2Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France
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  • ORCID record for Christophe Leterrier
  • For correspondence: s.vassilopoulos@institut-myologie.org christophe.leterrier@univ-amu.fr
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Abstract

Recent super-resolution microscopy studies have unveiled a periodic scaffold of actin rings regularly spaced by spectrins under the plasma membrane of axons. However, ultrastructural details are unknown, limiting a molecular and mechanistic understanding of these enigmatic structures. Here, we combine platinum-replica electron and optical super-resolution micros-copy to investigate the cortical cytoskeleton of axons at the ultrastructural level. We resolve actin rings as braids made of two long, intertwined actin filaments connected by a dense mesh of aligned spectrins. This molecular arrangement contrasts with the currently assumed model of actin rings made of short, capped actin filaments. We propose that braided rings explain the stability of the actin-spectrin scaffold and ultimately help preserving the axon integrity.

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Posted May 13, 2019.
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Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings
Stéphane Vassilopoulos, Solène Gibaud, Angélique Jimenez, Ghislaine Caillol, Christophe Leterrier
bioRxiv 636217; doi: https://doi.org/10.1101/636217
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Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings
Stéphane Vassilopoulos, Solène Gibaud, Angélique Jimenez, Ghislaine Caillol, Christophe Leterrier
bioRxiv 636217; doi: https://doi.org/10.1101/636217

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