Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Interactions between the gut microbiome and mucosal immunoglobulins A, M and G in the developing infant gut

Anders Janzon, Julia K. Goodrich, Omry Koren, the TEDDY Study Group, Jillian L. Waters, Ruth E. Ley
doi: https://doi.org/10.1101/637959
Anders Janzon
1Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julia K. Goodrich
1Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Omry Koren
1Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany
2Azrieli Faculty of Medicine, Bar Ilan University, Safed 1311502, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jillian L. Waters
1Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruth E. Ley
1Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rley@tuebingen.mpg.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Objective Interactions between the gut microbiome and immunoglobulin (Ig) A in infancy are important for future health. IgM and IgG are also present, however, their interactions with the microbiome in the developing infant are less understood.

Design We employed stool samples sampled 15 times in infancy from 32 healthy subjects at 4 locations in 3 countries (from the TEDDY study). We characterized patterns of microbiome development in relation to levels of IgA, IgG and IgM. For 8 infants from a single location, we FACS-sorted microbial cells from stool by Ig status. We used 16S rRNA gene profiling on full and sorted microbiomes to assess patterns of antibody coating in relation to age and other factors.

Results All antibodies decreased in concentration with age, but were augmented by breastmilk feeding regardless of infant age. Levels of IgA correlated with the relative abundances of OTUs belonging to the Bifidobacteria and Enterobacteriaceae, which dominated the early microbiome, and IgG levels correlated with Haemophilus. The diversity of Ig-coated microbiota was influenced by breastfeeding and age, but birth mode. IgA and IgM coated the same microbiota, while IgG targeted a different subset. Blautia generally evaded antibody coating, while members of the Bifidobacteria and Enterobacteriaceae were high in IgA/M.

Conclusion IgA/M have similar dynamics with respect to microbiome development with age, and their interactions with the microbiome are influenced by breastfeeding status. IgG generally does not coat the commensal microbiota.

Summary What is already known on this subject?

  • Secretory IgA coats ~50% of microbiota in the gut

  • IgM and IgG are less prevalent and coat a lower fraction in the adult, dynamics in the infant gut are not well characterized.

  • Breastmilk is a source of IgA to the infant gut and decreases with time.

  • IgA coating of microbial cells in infant gut microbiome decreases over time.

What are the new findings?

  • Breastfeeding augments the IgA coating of the microbiome at all ages.

  • IgA and IgM coat many of the same cells, whereas few are coated by IgG alone.

  • Bifidobacteria, Enterobacteriaceae, Ruminococcus gnavus are enriched in IgA/M-coated cell fraction, Blautia is enriched in uncoated fraction.

  • IgG levels correlated with Haemophilus.

How might it impact on clinical practice in the foreseeable future?

  • Ig-coated fraction of the gut microbiome could serve as a useful tool for tracking development of the infant gut microbiome, and/or identifying aberrations to immune sensing of the microbiome.

Footnotes

  • ↵* co-first authors

  • ↵^ Members of the TEDDY Study Group are listed in the Supplemental Online Appendix.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 15, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Interactions between the gut microbiome and mucosal immunoglobulins A, M and G in the developing infant gut
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Interactions between the gut microbiome and mucosal immunoglobulins A, M and G in the developing infant gut
Anders Janzon, Julia K. Goodrich, Omry Koren, the TEDDY Study Group, Jillian L. Waters, Ruth E. Ley
bioRxiv 637959; doi: https://doi.org/10.1101/637959
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Interactions between the gut microbiome and mucosal immunoglobulins A, M and G in the developing infant gut
Anders Janzon, Julia K. Goodrich, Omry Koren, the TEDDY Study Group, Jillian L. Waters, Ruth E. Ley
bioRxiv 637959; doi: https://doi.org/10.1101/637959

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3514)
  • Biochemistry (7367)
  • Bioengineering (5347)
  • Bioinformatics (20326)
  • Biophysics (10046)
  • Cancer Biology (7777)
  • Cell Biology (11353)
  • Clinical Trials (138)
  • Developmental Biology (6453)
  • Ecology (9980)
  • Epidemiology (2065)
  • Evolutionary Biology (13357)
  • Genetics (9373)
  • Genomics (12614)
  • Immunology (7725)
  • Microbiology (19104)
  • Molecular Biology (7465)
  • Neuroscience (41153)
  • Paleontology (301)
  • Pathology (1235)
  • Pharmacology and Toxicology (2142)
  • Physiology (3180)
  • Plant Biology (6880)
  • Scientific Communication and Education (1276)
  • Synthetic Biology (1900)
  • Systems Biology (5328)
  • Zoology (1091)