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Genome replication dynamics of a bacteriophage and its satellite reveal strategies for parasitism and viral restriction

Zachary K Barth, Tania V Silvas, Angus Angermeyer, View ORCID ProfileKimberley D Seed
doi: https://doi.org/10.1101/639039
Zachary K Barth
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Tania V Silvas
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Angus Angermeyer
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Kimberley D Seed
1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Chan Zuckerberg Biohub, San Francisco, CA 94158, USA
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  • ORCID record for Kimberley D Seed
  • For correspondence: kseed@berkeley.edu
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ABSTRACT

Phage-inducible chromosomal island-like elements (PLEs) are bacteriophage satellites found in Vibrio cholerae. PLEs parasitize the lytic phage ICP1, excising from the bacterial chromosome, replicating, and mobilizing to new host cells following cell lysis. PLEs protect their host cell populations by completely restricting the production of ICP1 progeny. Previously, it was found that ICP1 replication was reduced during PLE(+) infection. Despite robustly replicating its genome, PLE produces relatively few transducing units, leading us to investigate if PLE DNA replication itself is antagonistic to ICP1 replication. Here we identify key constituents of PLE replication and assess their role in interference of ICP1. PLE encodes a RepA_N initiation factor that is sufficient to drive replication from the PLE origin of replication during ICP1 infection. In contrast to previously characterized bacteriophage satellites, expression of the PLE initiation factor was not sufficient for PLE replication in the absence of phage. Replication of PLE was necessary for interference of ICP1 DNA replication, but replication of a minimalized PLE replicon was not sufficient for ICP1 DNA replication interference. Despite restoration of ICP1 DNA replication, non-replicating PLE remained broadly inhibitory against ICP1. These results suggest that PLE DNA replication is one of multiple mechanisms contributing to ICP1 restriction.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 17, 2019.
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Genome replication dynamics of a bacteriophage and its satellite reveal strategies for parasitism and viral restriction
Zachary K Barth, Tania V Silvas, Angus Angermeyer, Kimberley D Seed
bioRxiv 639039; doi: https://doi.org/10.1101/639039
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Genome replication dynamics of a bacteriophage and its satellite reveal strategies for parasitism and viral restriction
Zachary K Barth, Tania V Silvas, Angus Angermeyer, Kimberley D Seed
bioRxiv 639039; doi: https://doi.org/10.1101/639039

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