Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Actin chromobody imaging reveals sub-organellar actin dynamics

View ORCID ProfileCara Schiavon, Tong Zhang, Bing Zhao, View ORCID ProfileLeonardo Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn, Jasmine W. Feng, View ORCID ProfileOmar A. Quintero, View ORCID ProfileRobert Grosse, View ORCID ProfileUri Manor
doi: https://doi.org/10.1101/639278
Cara Schiavon
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cara Schiavon
Tong Zhang
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bing Zhao
2Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leonardo Andrade
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Leonardo Andrade
Melissa Wu
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsung-Chang Sung
3Transgenic Core, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yelena Dayn
3Transgenic Core, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jasmine W. Feng
4Department of Biology, University of Richmond, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Omar A. Quintero
4Department of Biology, University of Richmond, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Omar A. Quintero
Robert Grosse
3Transgenic Core, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robert Grosse
Uri Manor
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Uri Manor
  • For correspondence: umanor@salk.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are difficult to detect with fluorescence microscopy. We generated fluorescent protein-tagged actin nanobodies targeted to organelle membranes to enable live cell imaging of sub-organellar actin dynamics with high spatiotemporal resolution. These probes reveal ER-associated actin drives fission of multiple organelles including mitochondria, endosomes, lysosomes, peroxisomes, and the Golgi.

Figure
  • Download figure
  • Open in new tab
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 15, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Actin chromobody imaging reveals sub-organellar actin dynamics
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Actin chromobody imaging reveals sub-organellar actin dynamics
Cara Schiavon, Tong Zhang, Bing Zhao, Leonardo Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn, Jasmine W. Feng, Omar A. Quintero, Robert Grosse, Uri Manor
bioRxiv 639278; doi: https://doi.org/10.1101/639278
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Actin chromobody imaging reveals sub-organellar actin dynamics
Cara Schiavon, Tong Zhang, Bing Zhao, Leonardo Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn, Jasmine W. Feng, Omar A. Quintero, Robert Grosse, Uri Manor
bioRxiv 639278; doi: https://doi.org/10.1101/639278

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4115)
  • Biochemistry (8818)
  • Bioengineering (6522)
  • Bioinformatics (23466)
  • Biophysics (11792)
  • Cancer Biology (9212)
  • Cell Biology (13326)
  • Clinical Trials (138)
  • Developmental Biology (7439)
  • Ecology (11416)
  • Epidemiology (2066)
  • Evolutionary Biology (15155)
  • Genetics (10439)
  • Genomics (14045)
  • Immunology (9173)
  • Microbiology (22160)
  • Molecular Biology (8814)
  • Neuroscience (47582)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3731)
  • Plant Biology (8082)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1253)