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Systematic interrogation of the distinct metabolic potential in gut microbiomes of inflammatory bowel disease patients with dysbiosis

Almut Heinken, View ORCID ProfileInes Thiele
doi: https://doi.org/10.1101/640649
Almut Heinken
1School of Medicine, National University of Ireland, Galway, University Road, Galway, Ireland
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Ines Thiele
1School of Medicine, National University of Ireland, Galway, University Road, Galway, Ireland
2Discipline of Microbiology, School of Natural Sciences, National University of Ireland, Galway, University Road, Galway, Ireland
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  • ORCID record for Ines Thiele
  • For correspondence: ines.thiele@nuigalway.ie
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Abstract

The human gut microbiome plays an important role in human health. In order to investigate changes in metabolic activity associated with dysbiotic microbiomes, we retrieved strain-level relative abundances from metagenomics data from a cohort of pediatric Crohn’s Disease patients with and without dysbiosis, and healthy control children to construct a personalized microbiome model for each sample using the AGORA resource of genome-scale gut microbial reconstructions. Subsequently, we systematically profiled each individual microbiome by predicting the quantitative biosynthesis potential for all secreted metabolites as well as the strain-level contributions to each metabolite in each individual microbiome. The predicted fecal metabolomes and strain-metabolite contributions of microbiomes from patients with dysbiosis were distinct from healthy controls and patients without dysbiosis. Finally, we validated the predicted amino acid production profiles against fecal metabolomic measurements. Taken together, we presented an efficient, scalable, tractable computational approach to systematically interrogate the metabolic potential of individual microbiomes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 17, 2019.
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Systematic interrogation of the distinct metabolic potential in gut microbiomes of inflammatory bowel disease patients with dysbiosis
Almut Heinken, Ines Thiele
bioRxiv 640649; doi: https://doi.org/10.1101/640649
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Systematic interrogation of the distinct metabolic potential in gut microbiomes of inflammatory bowel disease patients with dysbiosis
Almut Heinken, Ines Thiele
bioRxiv 640649; doi: https://doi.org/10.1101/640649

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