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Low-cost, open-access refractive index mapping of algal cells using the transport of intensity equation

Stephen Grant, Kyle Richford, Heidi Burdett, David McKee, Brian R. Patton
doi: https://doi.org/10.1101/640755
Stephen Grant
1Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom
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  • For correspondence: stephen.d.grant@strath.ac.uk
Kyle Richford
1Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom
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Heidi Burdett
2Lyell Centre for Earth and Marine Science and Technology, Edinburgh, UK, EH14 4AS
3School of Energy, Geoscience, Infrastructure and Society, Heriot-Watt University, Edinburgh, EH14 4AP
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David McKee
1Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom
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Brian R. Patton
1Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom
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Abstract

Phase contrast microscopy allows stain free imaging of transparent biological samples. One technique, using the transport of intensity equation (TIE), can be performed without dedicated hardware by simply processing pairs of images taken at known spacings within the sample. The resulting TIE images are quantitative phase maps of unstained biological samples. Therefore, spatially resolved refractive index information can also be determined.

Using low-cost, open-source hardware, we applied the TIE to living algal cells to measure their refractive index. We obtained refractive index values that were repeatable within species and differed by distinct amounts depending on the species being measured. We suggest TIE imaging as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index. Potential applications in biogeochemical modelling and climate sciences are suggested.

Footnotes

  • https://strathcloud.sharefile.eu/share/view/s7ea19eb4f6b4730a/fo340526-6674-4766-9478-d7d64835a190

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 17, 2019.
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Low-cost, open-access refractive index mapping of algal cells using the transport of intensity equation
Stephen Grant, Kyle Richford, Heidi Burdett, David McKee, Brian R. Patton
bioRxiv 640755; doi: https://doi.org/10.1101/640755
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Low-cost, open-access refractive index mapping of algal cells using the transport of intensity equation
Stephen Grant, Kyle Richford, Heidi Burdett, David McKee, Brian R. Patton
bioRxiv 640755; doi: https://doi.org/10.1101/640755

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