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Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light

View ORCID ProfileAretha Fiebig, Lydia M. Varesio, Xiomarie Alejandro Navarreto, View ORCID ProfileSean Crosson
doi: https://doi.org/10.1101/641647
Aretha Fiebig
1Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637
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  • For correspondence: amfiebig@uchicago.edu scrosson@uchicago.edu
Lydia M. Varesio
2The Committee on Microbiology, The University of Chicago, Chicago, IL 60637
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Xiomarie Alejandro Navarreto
1Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637
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Sean Crosson
1Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637
2The Committee on Microbiology, The University of Chicago, Chicago, IL 60637
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  • ORCID record for Sean Crosson
  • For correspondence: amfiebig@uchicago.edu scrosson@uchicago.edu
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SUMMARY

Extracytoplasmic function (ECF) sigma factors are a major class of environmentally-responsive transcriptional regulators. In Alphaproteobacteria the ECF sigma factor, σEcfG, activates general stress response (GSR) transcription and protects cells from multiple stressors. A phosphorylation-dependent protein partner switching mechanism, involving HWE/HisKA_2-family histidine kinases, underlies σEcfG activation. The identity of these sensor kinases and the signals that regulate them remain largely uncharacterized. We have developed the aerobic anoxygenic photoheterotrophic (AAP) bacterium, Erythrobacter litoralis DSM 8509, as a comparative genetic model to investigate GSR regulation. Using this system, we sought to define the contribution of visible light and a photosensory HWE kinase, LovK, to GSR transcription. We identified three HWE kinases that collectively regulate GSR: gsrK and lovK are activators, while gsrP is a repressor. GSR transcription is higher in the dark than light, and the opposing activities of gsrK and gsrP are sufficient to achieve light-dependent differential transcription. In the absence of gsrK and gsrP, lovK alone is sufficient to regulate GSR transcription in response to light. This regulation requires a photochemically active LOV domain in LovK. Our studies establish a role for visible light and HWE kinases in light-dependent regulation of GSR transcription in E. litoralis, an AAP species.

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ABBREVIATED SUMMARY General stress response (GSR) systems protect bacteria from a diverse range of physical and chemical stressors. We have developed Erythrobacter litoralis as a new genetic model to study GSR in Alphaproteobacteria and show that three HWE-family histidine kinases collectively regulate GSR transcription via σEcfG. Visible light is a GSR regulatory signal in E. litoralis, and LovK is a blue-light photosensor kinase that functions as a dark activated GSR regulator.

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  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126532

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 19, 2019.
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Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light
Aretha Fiebig, Lydia M. Varesio, Xiomarie Alejandro Navarreto, Sean Crosson
bioRxiv 641647; doi: https://doi.org/10.1101/641647
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Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light
Aretha Fiebig, Lydia M. Varesio, Xiomarie Alejandro Navarreto, Sean Crosson
bioRxiv 641647; doi: https://doi.org/10.1101/641647

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