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Culturable diversity of Arctic phytoplankton during pack ice melting

View ORCID ProfileCatherine Gérikas Ribeiro, View ORCID ProfileAdriana Lopes dos Santos, Priscillia Gourvil, Florence Le Gall, Dominique Marie, Margot Tragin, View ORCID ProfileIan Probert, View ORCID ProfileDaniel Vaulot
doi: https://doi.org/10.1101/642264
Catherine Gérikas Ribeiro
1Sorbonne Université, CNRS, UMR7144, Team ECOMAP, Station Biologique de Roscoff, Roscoff, France
2GEMA Center for Genomics, Ecology & Environment, Universidad Mayor, Camino La Pirámide, 5750, Huechuraba, Santiago, Chile
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  • For correspondence: catherine.gerikas@gmail.com
Adriana Lopes dos Santos
3Nanyang Technological University, Asian School of the Environment, Singapore
2GEMA Center for Genomics, Ecology & Environment, Universidad Mayor, Camino La Pirámide, 5750, Huechuraba, Santiago, Chile
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Priscillia Gourvil
4Sorbonne Université, CNRS, FR2424, Roscoff Culture Collection, Station Biologique de Roscoff, Roscoff, France
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Florence Le Gall
1Sorbonne Université, CNRS, UMR7144, Team ECOMAP, Station Biologique de Roscoff, Roscoff, France
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Dominique Marie
1Sorbonne Université, CNRS, UMR7144, Team ECOMAP, Station Biologique de Roscoff, Roscoff, France
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Margot Tragin
1Sorbonne Université, CNRS, UMR7144, Team ECOMAP, Station Biologique de Roscoff, Roscoff, France
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Ian Probert
4Sorbonne Université, CNRS, FR2424, Roscoff Culture Collection, Station Biologique de Roscoff, Roscoff, France
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Daniel Vaulot
1Sorbonne Université, CNRS, UMR7144, Team ECOMAP, Station Biologique de Roscoff, Roscoff, France
3Nanyang Technological University, Asian School of the Environment, Singapore
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Abstract

Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 genotypes defined by 100% 18S rRNA sequence similarity. These genotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Rhodomonas and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis.

Submitted to: Elementa: Science of the Anthropocene Date: May 17, 2019

Footnotes

  • https://github.com/vaulot/Paper-2019-Ribeiro-GE-cultures

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 20, 2019.
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Culturable diversity of Arctic phytoplankton during pack ice melting
Catherine Gérikas Ribeiro, Adriana Lopes dos Santos, Priscillia Gourvil, Florence Le Gall, Dominique Marie, Margot Tragin, Ian Probert, Daniel Vaulot
bioRxiv 642264; doi: https://doi.org/10.1101/642264
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Culturable diversity of Arctic phytoplankton during pack ice melting
Catherine Gérikas Ribeiro, Adriana Lopes dos Santos, Priscillia Gourvil, Florence Le Gall, Dominique Marie, Margot Tragin, Ian Probert, Daniel Vaulot
bioRxiv 642264; doi: https://doi.org/10.1101/642264

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