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Expression of c-Fos and Arc in hippocampal region CA1 marks neurons that exhibit learning-related activity changes

David Mahringer, Anders V. Petersen, Aris Fiser, Hiroyuki Okuno, Haruhiko Bito, Jean-François Perrier, Georg B. Keller
doi: https://doi.org/10.1101/644526
David Mahringer
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2Faculty of Natural Sciences, University of Basel, Basel, Switzerland
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Anders V. Petersen
3Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark
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Aris Fiser
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
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Hiroyuki Okuno
4Department of Biochemistry and Molecular Biology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima 890-8544, Japan.
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Haruhiko Bito
5Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
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Jean-François Perrier
3Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark
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Georg B. Keller
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2Faculty of Natural Sciences, University of Basel, Basel, Switzerland
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  • For correspondence: georg.keller@fmi.ch
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Abstract

Immediate early genes (IEGs) are transcribed in response to neural activity and necessary for many forms of plasticity. However, the dynamics of their expression during learning, as well as their relationship to neural activity, remain unclear. Here we used two-photon imaging in transgenic mice that express a GFP-tagged variant of Arc or c-Fos and a red-shifted calcium indicator to measure learning-related changes in IEG expression levels and neural activity in hippocampal region CA1 as mice learned to perform a two-alternative forced choice task. Neural activity levels correlated positively with IEG expression levels in vivo. In addition, we found that with learning, a subset of neurons in CA1 increased their responses to the reward-predicting cue, and IEG expression levels early in learning were selectively increased in neurons that would exhibit the strongest learning-related changes. Our findings are consistent with an interpretation of IEG expression levels as markers for experience dependent plasticity.

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Posted May 23, 2019.
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Expression of c-Fos and Arc in hippocampal region CA1 marks neurons that exhibit learning-related activity changes
David Mahringer, Anders V. Petersen, Aris Fiser, Hiroyuki Okuno, Haruhiko Bito, Jean-François Perrier, Georg B. Keller
bioRxiv 644526; doi: https://doi.org/10.1101/644526
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Expression of c-Fos and Arc in hippocampal region CA1 marks neurons that exhibit learning-related activity changes
David Mahringer, Anders V. Petersen, Aris Fiser, Hiroyuki Okuno, Haruhiko Bito, Jean-François Perrier, Georg B. Keller
bioRxiv 644526; doi: https://doi.org/10.1101/644526

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