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Characterization of three Yarrowia lipolytica strains in respect to different cultivation temperatures and metabolite secretion

S Hackenschmidt, F Bracharz, R Daniel, A Thürmer, S Bruder, J Kabisch
doi: https://doi.org/10.1101/645242
S Hackenschmidt
1Technische Universität Darmstadt, Computergestützte Synthetische Biologie, Schnittspahnstr. 10, 64287 Darmstadt, Germany
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F Bracharz
1Technische Universität Darmstadt, Computergestützte Synthetische Biologie, Schnittspahnstr. 10, 64287 Darmstadt, Germany
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R Daniel
2Georg-August University Göttingen, Institute of Microbiology and Genetics, Department of Genomic and Applied Microbiology, Grisebachstr. 8, 37077 Göttingen, Germany
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A Thürmer
3Robert Koch Institute Berlin, Seestrasse 10, 13353 Berlin, Germany
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S Bruder
1Technische Universität Darmstadt, Computergestützte Synthetische Biologie, Schnittspahnstr. 10, 64287 Darmstadt, Germany
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J Kabisch
1Technische Universität Darmstadt, Computergestützte Synthetische Biologie, Schnittspahnstr. 10, 64287 Darmstadt, Germany
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  • For correspondence: johannes@kabisch-lab.de
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Abstract

Despite the increasing relevance, ranging from academic research to industrial applications, only a limited number of nonconventional, oleaginous Yarrowia lipolytica strains are characterized in detail. Therefore, we analyzed three strains in regard to their metabolic and physiological properties and in respect to important characteristics of a production strains. A flow cytometry method was set up to evaluate their fitness in a rapid manner. By investigating different cultivation conditions and media compositions, similarities and differences between the distinct strain backgrounds could be derived. Especially sugar alcohol production, as well as a agglomeration of cells were found to be connected with growth at high temperatures. In addition, sugar alcohol production was independent of high substrate concentrations under these conditions. To investigate particular traits, including growth characteristics and metabolite concentrations, genomic analysis were performed. We found sequence variations for one third of the annotated proteins but no obvious link to all phenotypic features.

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Posted May 23, 2019.
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Characterization of three Yarrowia lipolytica strains in respect to different cultivation temperatures and metabolite secretion
S Hackenschmidt, F Bracharz, R Daniel, A Thürmer, S Bruder, J Kabisch
bioRxiv 645242; doi: https://doi.org/10.1101/645242
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Characterization of three Yarrowia lipolytica strains in respect to different cultivation temperatures and metabolite secretion
S Hackenschmidt, F Bracharz, R Daniel, A Thürmer, S Bruder, J Kabisch
bioRxiv 645242; doi: https://doi.org/10.1101/645242

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