Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

PfCERLI1, a conserved rhoptry associated protein essential for invasion by Plasmodium falciparum merozoites

View ORCID ProfileBenjamin Liffner, Sonja Frölich, Gary K. Heinemann, View ORCID ProfileBoyin Liu, View ORCID ProfileMatthew W. A. Dixon, Tim-Wolf Gilberger, View ORCID ProfileDanny W. Wilson
doi: https://doi.org/10.1101/646299
Benjamin Liffner
1Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benjamin Liffner
Sonja Frölich
1Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gary K. Heinemann
2Experimental Therapeutics Laboratory, School of Pharmacy & Medical Sciences, University of South Australia Cancer Research Institute, Adelaide, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Boyin Liu
3Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Boyin Liu
Matthew W. A. Dixon
3Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matthew W. A. Dixon
Tim-Wolf Gilberger
4Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
5Centre for Structural Systems Biology, 22607 Hamburg, Germany
6Biology Department, University of Hamburg, D-20146, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Danny W. Wilson
1Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, Australia
7Burnet Institute, 85 Commercial Road, Melbourne 3004, Victoria, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Danny W. Wilson
  • For correspondence: Danny.wilson@adelaide.edu.au
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The disease-causing blood stage of the Plasmodium falciparum lifecycle begins with invasion of human erythrocytes by merozoites. Many vaccine candidates with key roles in binding to the erythrocyte surface and entry are secreted from the large bulb-like rhoptry organelles at the apical tip of the merozoite. Here we identify an essential role for the conserved protein P. falciparum Cytosolically Exposed Rhoptry Leaflet Interacting protein 1 (PfCERLI1) in rhoptry function. We show that PfCERLI1 localises to the cytosolic face of the rhoptry bulb membrane and knockdown of PfCERLI1 inhibits merozoite invasion. While schizogony and merozoite organelle biogenesis appear normal, biochemical techniques and semi-quantitative super-resolution microscopy show that PfCERLI1 knockdown prevents secretion of key rhoptry antigens that coordinate merozoite invasion. PfCERLI1 is the first rhoptry associated protein identified to have a direct role in function of this essential malaria invasion organelle which has broader implications for understanding apicomplexan invasion biology.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 24, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
PfCERLI1, a conserved rhoptry associated protein essential for invasion by Plasmodium falciparum merozoites
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
PfCERLI1, a conserved rhoptry associated protein essential for invasion by Plasmodium falciparum merozoites
Benjamin Liffner, Sonja Frölich, Gary K. Heinemann, Boyin Liu, Matthew W. A. Dixon, Tim-Wolf Gilberger, Danny W. Wilson
bioRxiv 646299; doi: https://doi.org/10.1101/646299
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
PfCERLI1, a conserved rhoptry associated protein essential for invasion by Plasmodium falciparum merozoites
Benjamin Liffner, Sonja Frölich, Gary K. Heinemann, Boyin Liu, Matthew W. A. Dixon, Tim-Wolf Gilberger, Danny W. Wilson
bioRxiv 646299; doi: https://doi.org/10.1101/646299

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4091)
  • Biochemistry (8772)
  • Bioengineering (6487)
  • Bioinformatics (23356)
  • Biophysics (11756)
  • Cancer Biology (9154)
  • Cell Biology (13257)
  • Clinical Trials (138)
  • Developmental Biology (7418)
  • Ecology (11376)
  • Epidemiology (2066)
  • Evolutionary Biology (15095)
  • Genetics (10403)
  • Genomics (14014)
  • Immunology (9126)
  • Microbiology (22070)
  • Molecular Biology (8783)
  • Neuroscience (47395)
  • Paleontology (350)
  • Pathology (1421)
  • Pharmacology and Toxicology (2482)
  • Physiology (3705)
  • Plant Biology (8054)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2211)
  • Systems Biology (6017)
  • Zoology (1250)