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Primordial Blasts, a population of blood borne stem cells responsible for whole body regeneration in a basal chordate

View ORCID ProfileSusannah H. Kassmer, View ORCID ProfileAdam Langenbacher, Anthony W. De Tomaso
doi: https://doi.org/10.1101/647578
Susannah H. Kassmer
1Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA USA
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  • ORCID record for Susannah H. Kassmer
  • For correspondence: susannahhelene@gmail.com
Adam Langenbacher
2Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA USA
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Anthony W. De Tomaso
1Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA USA
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Abstract

The invertebrate chordate Botrylloides leachii regenerates entire new bodies from small fragments of blood vessels in a process called whole body regeneration (WBR). During the early stages of WBR, proliferation occurs only in small, blood borne cells expressing integrin-alpha-6, pou3 and vasa. Proliferating blood cells can be ablated using Mitomycin C (MMC), and MMC treatment blocks regeneration. However, regeneration could be rescued by injection of blood borne cells from healthy individuals, and injection of a single Integrin-alpha-6 (IA6)-positive cell is capable of rescuing whole body regeneration. Following transplantation of EdU-labeled IA6+ cells into MMC treated recipients, EdU label is detected in regenerating tissues. Our data suggest that ia6+pou3+ cells are proliferating blood borne stem cells that are responsible for whole body regeneration, and we named these cells Primordial Blasts. Primordial Blasts express notch and frizzled receptors, and inhibitors of either Notch or canonical Wnt signaling block regeneration and proliferation of Primordial Blasts.

Footnotes

  • Susannah.kassmer{at}lifesci.ucsb.edu

  • added one missing reference, added primer sequences and additional methods to main text

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 29, 2019.
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Primordial Blasts, a population of blood borne stem cells responsible for whole body regeneration in a basal chordate
Susannah H. Kassmer, Adam Langenbacher, Anthony W. De Tomaso
bioRxiv 647578; doi: https://doi.org/10.1101/647578
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Primordial Blasts, a population of blood borne stem cells responsible for whole body regeneration in a basal chordate
Susannah H. Kassmer, Adam Langenbacher, Anthony W. De Tomaso
bioRxiv 647578; doi: https://doi.org/10.1101/647578

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