Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

cfrB, cfrC, and a potential new cfr-like gene in Clostridium difficile strains recovered across Latin America

Vanja Stojković, María Fernanda Ulate, Fanny Hidalgo-Villeda, Emmanuel Aguilar, Camilo Monge-Cascante, Marjorie Pizarro-Guajardo, Kaitlyn Tsai, Edgardo Tzoc, Margarita Camorlinga, Daniel Paredes-Sabja, View ORCID ProfileCarlos Quesada-Gómez, Danica Galonić Fujimori, View ORCID ProfileCésar Rodríguez
doi: https://doi.org/10.1101/649020
Vanja Stojković
1Department of Cellular and Molecular Pharmacology, University of California San Francisco, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
María Fernanda Ulate
2CIET and Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fanny Hidalgo-Villeda
3Escuela de Microbiología, Facultad de Ciencias, Universidad Nacional Autónoma de Honduras, Honduras
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emmanuel Aguilar
4Medical Bacteriology, Department of Microbiology. Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional (ENCB-IPN), México
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Camilo Monge-Cascante
2CIET and Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marjorie Pizarro-Guajardo
6Microbiota-Host Interactions and Clostridia Research Group, Facultad Ciencias de la Vida, Universidad Andres Bello, Chile
7Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaitlyn Tsai
1Department of Cellular and Molecular Pharmacology, University of California San Francisco, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edgardo Tzoc
3Escuela de Microbiología, Facultad de Ciencias, Universidad Nacional Autónoma de Honduras, Honduras
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Margarita Camorlinga
5Unidad de Investigación en Enfermedades Infecciosas y Parasitarias. Instituto Mexicano del Seguro Social, México
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Paredes-Sabja
6Microbiota-Host Interactions and Clostridia Research Group, Facultad Ciencias de la Vida, Universidad Andres Bello, Chile
7Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carlos Quesada-Gómez
2CIET and Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carlos Quesada-Gómez
Danica Galonić Fujimori
1Department of Cellular and Molecular Pharmacology, University of California San Francisco, USA
8Department of Pharmaceutical Chemistry, University of California San Francisco, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
César Rodríguez
2CIET and Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for César Rodríguez
  • For correspondence: cesar.rodriguezsanchez@ucr.ac.cr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Cfr is a radical S-adenosyl-L-methionine (SAM) enzyme that confers cross-resistance to all antibiotics targeting the large ribosomal subunit through hypermethylation of nucleotide A2503 of 23S rRNA. Of the four known cfr genes known to date, cfr(B) and cfr(C) have been sporadically found in C. difficile, yet functional characterization of cfr(C) is still lacking. We identified genes for putative Cfr-like enzymes among clinical C. difficile strains from Mexico, Honduras, Costa Rica, and Chile. To confirm their identity and activity, we obtained minimum inhibitory concentrations for ribosome-targeting antibiotics, annotated whole genome sequences, and performed a functional characterization of Cfr(C). The seven representative isolates analyzed displayed different levels of resistance to PhLOPSA antibiotics in the absence of the ribosome protection factor OptrA, and mutations in genes for 23S rRNAs or the ribosomal proteins L3 and L4. cfr(B) was detected in four isolates as part of a Tn6218-like transposon or an un-described mobile genetic element. In turn, cfr(C) was found integrated into an ICE-element. One isolate harbored a putative cfr-like gene that shows only 51-58% of sequence identity to Cfr and known Cfr-like enzymes. Moreover, our in vitro assays confirmed that Cfr(C) methylates E. coli and C. difficile 23S rRNA fragments. These results indicate selection of cfr-like genes in C. difficile from Latin America, suggest that the diversity of cfr-like resistance genes is larger than anticipated, and provide the first assessment of the methylation activity of Cfr(C).

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 24, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
cfrB, cfrC, and a potential new cfr-like gene in Clostridium difficile strains recovered across Latin America
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
cfrB, cfrC, and a potential new cfr-like gene in Clostridium difficile strains recovered across Latin America
Vanja Stojković, María Fernanda Ulate, Fanny Hidalgo-Villeda, Emmanuel Aguilar, Camilo Monge-Cascante, Marjorie Pizarro-Guajardo, Kaitlyn Tsai, Edgardo Tzoc, Margarita Camorlinga, Daniel Paredes-Sabja, Carlos Quesada-Gómez, Danica Galonić Fujimori, César Rodríguez
bioRxiv 649020; doi: https://doi.org/10.1101/649020
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
cfrB, cfrC, and a potential new cfr-like gene in Clostridium difficile strains recovered across Latin America
Vanja Stojković, María Fernanda Ulate, Fanny Hidalgo-Villeda, Emmanuel Aguilar, Camilo Monge-Cascante, Marjorie Pizarro-Guajardo, Kaitlyn Tsai, Edgardo Tzoc, Margarita Camorlinga, Daniel Paredes-Sabja, Carlos Quesada-Gómez, Danica Galonić Fujimori, César Rodríguez
bioRxiv 649020; doi: https://doi.org/10.1101/649020

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4688)
  • Biochemistry (10379)
  • Bioengineering (7695)
  • Bioinformatics (26372)
  • Biophysics (13547)
  • Cancer Biology (10721)
  • Cell Biology (15460)
  • Clinical Trials (138)
  • Developmental Biology (8509)
  • Ecology (12842)
  • Epidemiology (2067)
  • Evolutionary Biology (16885)
  • Genetics (11416)
  • Genomics (15493)
  • Immunology (10638)
  • Microbiology (25254)
  • Molecular Biology (10239)
  • Neuroscience (54587)
  • Paleontology (402)
  • Pathology (1671)
  • Pharmacology and Toxicology (2899)
  • Physiology (4355)
  • Plant Biology (9263)
  • Scientific Communication and Education (1588)
  • Synthetic Biology (2561)
  • Systems Biology (6789)
  • Zoology (1470)