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Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection

Jenny Bottek, Camille Soun, Julia K Volke, Akanksha Dixit, Stephanie Thiebes, Anna-Lena Beerlage, Marius Horstmann, Annett Urbanek, Julian Uszkoreit, Martin Eisenacher, Thilo Bracht, Barbara Sitek, Franziska Hoffmann, Nirojah Vijitha, Ferdinand von Eggeling, View ORCID ProfileDaniel R Engel
doi: https://doi.org/10.1101/649137
Jenny Bottek
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Camille Soun
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Julia K Volke
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Akanksha Dixit
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Stephanie Thiebes
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Anna-Lena Beerlage
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Marius Horstmann
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Annett Urbanek
2Leibniz Institute of Photonic Technology, 07743, Jena, Germany
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Julian Uszkoreit
3Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, 44801 Bochum, Germany
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Martin Eisenacher
3Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, 44801 Bochum, Germany
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Thilo Bracht
3Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, 44801 Bochum, Germany
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Barbara Sitek
3Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, 44801 Bochum, Germany
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Franziska Hoffmann
4Department of Otorhinolaryngology, Jena University Hospital, 07743, Jena, Germany
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Nirojah Vijitha
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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Ferdinand von Eggeling
4Department of Otorhinolaryngology, Jena University Hospital, 07743, Jena, Germany
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Daniel R Engel
1Institute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital Essen, 45147 Essen, Germany
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  • ORCID record for Daniel R Engel
  • For correspondence: engel@immunodynamics.de
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SUMMARY

Macrophages perform essential functions during bacterial infections, such as phagocytosis of pathogens and elimination of neutrophils to reduce spreading of infection, inflammation and tissue damage. The spatial distribution of macrophages is critical to respond to tissue specific adaptations upon infections. Using a novel algorithm for correlative mass spectrometry imaging and state-of-the-art multiplex microscopy, we report here that macrophages within the urinary bladder are positioned in the connective tissue underneath the urothelium. Invading uropathogenic E.coli induced an IL-6–dependent CX3CL1 expression by urothelial cells, facilitating relocation of macrophages from the connective tissue into the urothelium. These cells phagocytosed UPECs and eliminated neutrophils to maintain barrier function of the urothelium, preventing persistent and recurrent urinary tract infection.

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Posted June 06, 2019.
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Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection
Jenny Bottek, Camille Soun, Julia K Volke, Akanksha Dixit, Stephanie Thiebes, Anna-Lena Beerlage, Marius Horstmann, Annett Urbanek, Julian Uszkoreit, Martin Eisenacher, Thilo Bracht, Barbara Sitek, Franziska Hoffmann, Nirojah Vijitha, Ferdinand von Eggeling, Daniel R Engel
bioRxiv 649137; doi: https://doi.org/10.1101/649137
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Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection
Jenny Bottek, Camille Soun, Julia K Volke, Akanksha Dixit, Stephanie Thiebes, Anna-Lena Beerlage, Marius Horstmann, Annett Urbanek, Julian Uszkoreit, Martin Eisenacher, Thilo Bracht, Barbara Sitek, Franziska Hoffmann, Nirojah Vijitha, Ferdinand von Eggeling, Daniel R Engel
bioRxiv 649137; doi: https://doi.org/10.1101/649137

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