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Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum

Kati Böhm, Giacomo Giacomelli, Andreas Schmidt, Axel Imhof, Romain Koszul, Martial Marbouty, Marc Bramkamp
doi: https://doi.org/10.1101/649749
Kati Böhm
1Ludwig-Maximilians-Universität München, Fakultät Biologie, Großhaderner Straße 2-4, 82152 Planegg-Martinsried, Germany
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Giacomo Giacomelli
1Ludwig-Maximilians-Universität München, Fakultät Biologie, Großhaderner Straße 2-4, 82152 Planegg-Martinsried, Germany
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Andreas Schmidt
3Ludwig-Maximilians-Universität München, Zentrallabor für Proteinanalytik, Biomedizinisches Centrum München, 82152 Planegg-Martinsried, Germany
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Axel Imhof
3Ludwig-Maximilians-Universität München, Zentrallabor für Proteinanalytik, Biomedizinisches Centrum München, 82152 Planegg-Martinsried, Germany
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Romain Koszul
2Laboratoire Régulation Spatiale des Génomes, Institut Pasteur, UMR 3525, CNRS, 75015 Paris, France
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Martial Marbouty
2Laboratoire Régulation Spatiale des Génomes, Institut Pasteur, UMR 3525, CNRS, 75015 Paris, France
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  • For correspondence: marc.bramkamp@lmu.de martial.marbouty@pasteur.fr
Marc Bramkamp
1Ludwig-Maximilians-Universität München, Fakultät Biologie, Großhaderner Straße 2-4, 82152 Planegg-Martinsried, Germany
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  • For correspondence: marc.bramkamp@lmu.de martial.marbouty@pasteur.fr
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Abstract

Higher-order chromosome folding and segregation is tightly regulated in all domains of life. In bacteria, details on nucleoid organization regulatory mechanisms and function remains poorly characterized, especially in non-model species. Here, we investigate the role of DNA partitioning protein ParB and condensin complexes, two key players in bacterial chromosome structuring, in the actinobacterium Corynebacterium glutamicum. Chromosome conformation capture reveals SMC-mediated long-range interactions around ten centromere-like parS sites clustered at the replication origin (oriC). At least one oriC-proximal parS site is necessary for a reliable chromosome segregation. Using a combination of chromatin immunoprecipitation and photoactivated single molecule localization microscopy evidences the formation of distinct ParB-nucleoprotein subclusters in dependence of parS numbers. We further identified and functionally characterized two condensin paralogs. Whereas SMC/ScpAB complexes are loaded via ParB at parS sites mediating chromosomal inter-arm contacts like in Bacillus subtilis, the MukBEF-like SMC complex MksBEFG does not contribute to chromosomal DNA-folding. Rather, the MksBEFG complex is involved in plasmid maintenance and interacts with the polar oriC-tethering factor DivIVA. These data complement current models of ParB-SMC/ScpAB crosstalk, while showing that some condensin complexes evolved functions uncoupled from chromosome folding.

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Posted May 25, 2019.
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Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum
Kati Böhm, Giacomo Giacomelli, Andreas Schmidt, Axel Imhof, Romain Koszul, Martial Marbouty, Marc Bramkamp
bioRxiv 649749; doi: https://doi.org/10.1101/649749
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Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum
Kati Böhm, Giacomo Giacomelli, Andreas Schmidt, Axel Imhof, Romain Koszul, Martial Marbouty, Marc Bramkamp
bioRxiv 649749; doi: https://doi.org/10.1101/649749

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