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Promoters adopt distinct dynamic manifestations depending on transcription factor context

View ORCID ProfileAnders S. Hansen, View ORCID ProfileChristoph Zechner
doi: https://doi.org/10.1101/650762
Anders S. Hansen
1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
2Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
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  • ORCID record for Anders S. Hansen
  • For correspondence: anders.sejr.hansen@berkeley.edu zechner@mpi-cbg.de
Christoph Zechner
3Max Planck Institute of Molecular Cell Biology & Genetics, Germany
4Center for Systems Biology Dresden, Germany
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  • For correspondence: anders.sejr.hansen@berkeley.edu zechner@mpi-cbg.de
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Abstract

Cells respond to external signals and stresses by activating transcription factors (TF), which induce gene expression changes. Previous work suggests that signal-specific gene expression changes are partly achieved because different gene promoters exhibit varying induction dynamics in response to the same TF input signal. Here, using high-throughput quantitative single-cell measurements and a novel statistical method, we systematically analyzed transcription in individual cells to a large number of dynamic TF inputs. In particular, we quantified the scaling behavior among different transcriptional features extracted from the measured trajectories such as the gene activation delay or duration of promoter activity. Surprisingly, we found that even the same gene promoter can exhibit qualitatively distinct induction and scaling behaviors when exposed to different dynamic TF contexts. That is, promoters can adopt context-dependent “manifestations”. Our analysis suggests that the full complexity of signal processing by genetic circuits may be significantly underestimated when studied in specific contexts only.

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  • https://zenodo.org/record/2755026

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 26, 2019.
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Promoters adopt distinct dynamic manifestations depending on transcription factor context
Anders S. Hansen, Christoph Zechner
bioRxiv 650762; doi: https://doi.org/10.1101/650762
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Promoters adopt distinct dynamic manifestations depending on transcription factor context
Anders S. Hansen, Christoph Zechner
bioRxiv 650762; doi: https://doi.org/10.1101/650762

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