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Collective forces of tumor spheroids in three-dimensional biopolymer networks

Christoph Mark, Thomas J. Grundy, David Böhringer, Julian Steinwachs, Geraldine M. O’Neill, Ben Fabry
doi: https://doi.org/10.1101/654079
Christoph Mark
1Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
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  • For correspondence: christoph.mark@fau.de
Thomas J. Grundy
2Children’s Cancer Research Unit, The Children’s Hospital at Westmead, Westmead, Australia
3Discipline of Child and Adolescent Health, The University of Sydney
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David Böhringer
1Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
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Julian Steinwachs
1Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
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Geraldine M. O’Neill
2Children’s Cancer Research Unit, The Children’s Hospital at Westmead, Westmead, Australia
3Discipline of Child and Adolescent Health, The University of Sydney
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Ben Fabry
1Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
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ABSTRACT

We describe a method for quantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-dimensional collagen networks. While three-dimensional traction force microscopy for single cells in a nonlinear matrix is computationally complex due to the variable cell shape, here we exploit the spherical symmetry of tumor spheroids to derive a scale-invariant relationship between spheroid contractility and the surrounding matrix deformations. This relationship allows us to directly translate the magnitude of matrix deformations to the total contractility of arbitrarily sized spheroids. We show that collective forces of tumor spheroids reflect the contractility of individual cells for up to 1h after seeding, while collective forces on longer time-scales are guided by mechanical feedback from the extracellular matrix.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 31, 2019.
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Collective forces of tumor spheroids in three-dimensional biopolymer networks
Christoph Mark, Thomas J. Grundy, David Böhringer, Julian Steinwachs, Geraldine M. O’Neill, Ben Fabry
bioRxiv 654079; doi: https://doi.org/10.1101/654079
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Collective forces of tumor spheroids in three-dimensional biopolymer networks
Christoph Mark, Thomas J. Grundy, David Böhringer, Julian Steinwachs, Geraldine M. O’Neill, Ben Fabry
bioRxiv 654079; doi: https://doi.org/10.1101/654079

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