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Telophase correction refines division orientation in stratified epithelia

Kendall J. Lough, Kevin M. Byrd, Carlos P. Descovich, Danielle C. Spitzer, Abby J. Bergman, Gerard M. Beaudoin III, Louis F. Reichardt, Scott E. Williams
doi: https://doi.org/10.1101/668244
Kendall J. Lough
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
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Kevin M. Byrd
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
2Department of Oral & Craniofacial Health Sciences, University of North Carolina at Chapel Hill
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Carlos P. Descovich
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
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Danielle C. Spitzer
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
3Molecular and Cellular Biology Program, University of California, Berkeley
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Abby J. Bergman
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
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Gerard M. Beaudoin III
4Departments of Physiology and Biochemistry & Biophysics, University of California, San Francisco
5Department of Biology, Trinity University
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Louis F. Reichardt
4Departments of Physiology and Biochemistry & Biophysics, University of California, San Francisco
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Scott E. Williams
1Departments of Pathology & Laboratory Medicine and Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
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  • For correspondence: scott_williams@med.unc.edu
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ABSTRACT

During organogenesis, precise control of spindle orientation ensures a proper balance of proliferation and differentiation. In the developing murine epidermis, planar and perpendicular divisions yield symmetric and asymmetric fate outcomes, respectively. Classically, division axis specification involves centrosome migration and spindle rotation, events that occur early in mitosis. Here, we identify a previously uncharacterized orientation mechanism that occurs during telophase, correcting erroneous oblique orientations that unexpectedly persist into anaphase. The directionality of reorientation—towards either planar or perpendicular—correlates with the maintenance or loss of basal contact by the apical daughter. While the conserved scaffolding protein Pins/LGN is believed to function primarily through initial spindle positioning, we now show it also functions actively during telophase to reorient oblique divisions toward perpendicular. The ability to undergo telophase correction is also critically dependent upon an LGN-independent pathway involving the tension-sensitive adherens junction proteins vinculin, a-catenin and afadin, and correction directionality is influenced by local cell density. Failure of this reorientation mechanism impacts tissue architecture, as excessive oblique divisions induce precocious differentiation. The division orientation plasticity provided by telophase correction may provide a means for progenitors to dynamically respond to extrinsic cues provided by neighboring cells in order to adapt to local tissue needs.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 13, 2019.
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Telophase correction refines division orientation in stratified epithelia
Kendall J. Lough, Kevin M. Byrd, Carlos P. Descovich, Danielle C. Spitzer, Abby J. Bergman, Gerard M. Beaudoin III, Louis F. Reichardt, Scott E. Williams
bioRxiv 668244; doi: https://doi.org/10.1101/668244
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Telophase correction refines division orientation in stratified epithelia
Kendall J. Lough, Kevin M. Byrd, Carlos P. Descovich, Danielle C. Spitzer, Abby J. Bergman, Gerard M. Beaudoin III, Louis F. Reichardt, Scott E. Williams
bioRxiv 668244; doi: https://doi.org/10.1101/668244

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