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The ATPase cycle of Human Muscle Myosin II Isoforms: Adaptation of a single mechanochemical cycle for different physiological roles

Chloe A. Johnson, Jonathan Walklate, Marina Svicevic, Srboljub M. Mijailovich, Carlos Vera, Anastasia Karabina, Leslie A. Leinwand, Michael A. Geeves
doi: https://doi.org/10.1101/672030
Chloe A. Johnson
1School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
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Jonathan Walklate
1School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
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Marina Svicevic
2Faculty of Science, University of Kagujevac, Serbia
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Srboljub M. Mijailovich
3Department of Biology, Illinois Institute of Technology, Chicago IL 60616 USA
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Carlos Vera
4BioFrontiers Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder CO 80309 USA
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Anastasia Karabina
4BioFrontiers Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder CO 80309 USA
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Leslie A. Leinwand
4BioFrontiers Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder CO 80309 USA
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  • For correspondence: m.a.geeves@kent.ac.uk leslie.leinwand@colorado.edu
Michael A. Geeves
1School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
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  • For correspondence: m.a.geeves@kent.ac.uk leslie.leinwand@colorado.edu
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Abstract

Striated muscle myosins are encoded by a large gene family in all mammals, including human. These isoforms define several of the key characteristics of the different striated muscle fiber types including maximum shortening velocity. We have previously used recombinant isoforms of the motor domains of eight different human myosin isoforms to define the actin.myosin cross-bridge cycle in solution. Here, we use a recently developed modeling approach MUSICO to explore how well the experimentally defined cross-bridge cycles for each isoform in solution can predict the characteristics of muscle fiber contraction including duty ratio, shortening velocity, ATP economy and the load dependence of these parameters. The work shows that the parameters of the cross-bridge cycle predict many of the major characteristics of each muscle fiber type and raises the question of what sequence changes are responsible for these characteristics.

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Posted June 14, 2019.
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The ATPase cycle of Human Muscle Myosin II Isoforms: Adaptation of a single mechanochemical cycle for different physiological roles
Chloe A. Johnson, Jonathan Walklate, Marina Svicevic, Srboljub M. Mijailovich, Carlos Vera, Anastasia Karabina, Leslie A. Leinwand, Michael A. Geeves
bioRxiv 672030; doi: https://doi.org/10.1101/672030
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The ATPase cycle of Human Muscle Myosin II Isoforms: Adaptation of a single mechanochemical cycle for different physiological roles
Chloe A. Johnson, Jonathan Walklate, Marina Svicevic, Srboljub M. Mijailovich, Carlos Vera, Anastasia Karabina, Leslie A. Leinwand, Michael A. Geeves
bioRxiv 672030; doi: https://doi.org/10.1101/672030

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