Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Recombinant Expression of Aldehyde Dehydrogenase 2 (ALDH2) in Escherichia coli Nissle 1917 for Oral Delivery in ALDH2-Deficient Individuals

Tim Ho, Catherine Chang, Justin Wu, Iris Huang, Leona Tsai, Justin Lin, Emily Tai, Caroline Chou, Justin Yang, Yvonne Wei, Catherine Yeh, William Chen, Dylan Lu, Charlotte Chou, Longan Su, Nicole Chang, Colin Huang, Chloe Wang, Paul Abrena, Christy Cheung, Cassandra Yeh, Shantih Whiteford, Phoebe Chen, Austin Huang, Aire Wu, Benjamin Wei, Eugene Kao, Nicholas Lin, Anna Chang, Jake Yang, Yasmin Lin, Sean Tsao, Nicholas Ward, Teresa Chiang, Jude Clapper
doi: https://doi.org/10.1101/674606
Tim Ho
Taipei American School, Taipei City, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine Chang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justin Wu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Iris Huang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leona Tsai
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justin Lin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily Tai
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Chou
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justin Yang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yvonne Wei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine Yeh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William Chen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dylan Lu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Charlotte Chou
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Longan Su
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicole Chang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Colin Huang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chloe Wang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul Abrena
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christy Cheung
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cassandra Yeh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shantih Whiteford
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Phoebe Chen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Austin Huang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aire Wu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin Wei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eugene Kao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas Lin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Chang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jake Yang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasmin Lin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sean Tsao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas Ward
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Teresa Chiang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jude Clapper
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: clapperj@tas.edu.tw
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Data/Code
  • Preview PDF
Loading

Abstract

Turning red after consuming alcohol may seem like a mere social inconvenience. Yet, this flushing response is caused by an accumulation of acetaldehyde, a carcinogenic intermediate of alcohol metabolism. Aldehyde dehydrogenase 2 (ALDH2) deficiency, the result of a point mutation, produces a less efficient ALDH2. The resulting accumulation of acetaldehyde greatly increases the risk of developing esophageal and head and neck cancers. In this study, we produced recombinant ALDH2 in the probiotic E. coli Nissle 1917, which successfully reduces acetaldehyde levels in simulated oral conditions. Packaged in a hard candy, the ALDH2-probiotic would remain in the mouth to specifically target salivary acetaldehyde. Using mathematical modeling, we also determined how much recombinant ALDH2 is needed to reduce elevated acetaldehyde levels.

Financial Disclosure This work was funded by Taipei American School. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests The authors have declared that no competing interests exist.

Ethics Statement N/A

Data Availability Yes – all data are fully available without restriction. Sequences for the plasmids used in this study are available through the Registry of Standard Biological Parts. Links to raw data are included in Supplementary Information.

Footnotes

  • http://2018.igem.org/Team:TAS_Taipei

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 21, 2019.
Download PDF
Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Recombinant Expression of Aldehyde Dehydrogenase 2 (ALDH2) in Escherichia coli Nissle 1917 for Oral Delivery in ALDH2-Deficient Individuals
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Recombinant Expression of Aldehyde Dehydrogenase 2 (ALDH2) in Escherichia coli Nissle 1917 for Oral Delivery in ALDH2-Deficient Individuals
Tim Ho, Catherine Chang, Justin Wu, Iris Huang, Leona Tsai, Justin Lin, Emily Tai, Caroline Chou, Justin Yang, Yvonne Wei, Catherine Yeh, William Chen, Dylan Lu, Charlotte Chou, Longan Su, Nicole Chang, Colin Huang, Chloe Wang, Paul Abrena, Christy Cheung, Cassandra Yeh, Shantih Whiteford, Phoebe Chen, Austin Huang, Aire Wu, Benjamin Wei, Eugene Kao, Nicholas Lin, Anna Chang, Jake Yang, Yasmin Lin, Sean Tsao, Nicholas Ward, Teresa Chiang, Jude Clapper
bioRxiv 674606; doi: https://doi.org/10.1101/674606
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Recombinant Expression of Aldehyde Dehydrogenase 2 (ALDH2) in Escherichia coli Nissle 1917 for Oral Delivery in ALDH2-Deficient Individuals
Tim Ho, Catherine Chang, Justin Wu, Iris Huang, Leona Tsai, Justin Lin, Emily Tai, Caroline Chou, Justin Yang, Yvonne Wei, Catherine Yeh, William Chen, Dylan Lu, Charlotte Chou, Longan Su, Nicole Chang, Colin Huang, Chloe Wang, Paul Abrena, Christy Cheung, Cassandra Yeh, Shantih Whiteford, Phoebe Chen, Austin Huang, Aire Wu, Benjamin Wei, Eugene Kao, Nicholas Lin, Anna Chang, Jake Yang, Yasmin Lin, Sean Tsao, Nicholas Ward, Teresa Chiang, Jude Clapper
bioRxiv 674606; doi: https://doi.org/10.1101/674606

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Synthetic Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4672)
  • Biochemistry (10338)
  • Bioengineering (7658)
  • Bioinformatics (26300)
  • Biophysics (13501)
  • Cancer Biology (10672)
  • Cell Biology (15412)
  • Clinical Trials (138)
  • Developmental Biology (8487)
  • Ecology (12806)
  • Epidemiology (2067)
  • Evolutionary Biology (16831)
  • Genetics (11382)
  • Genomics (15469)
  • Immunology (10601)
  • Microbiology (25172)
  • Molecular Biology (10206)
  • Neuroscience (54383)
  • Paleontology (399)
  • Pathology (1667)
  • Pharmacology and Toxicology (2889)
  • Physiology (4334)
  • Plant Biology (9235)
  • Scientific Communication and Education (1586)
  • Synthetic Biology (2555)
  • Systems Biology (6773)
  • Zoology (1461)