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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

View ORCID ProfileBen J. Vincent, View ORCID ProfileGavin R. Rice, Gabriella M. Wong, View ORCID ProfileWilliam J. Glassford, Kayla I. Downs, Jessica L. Shastay, Kenechukwu Charles-Obi, Malini Natarajan, View ORCID ProfileMadelaine Gogol, View ORCID ProfileJulia Zeitlinger, View ORCID ProfileMark Rebeiz
doi: https://doi.org/10.1101/677260
Ben J. Vincent
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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Gavin R. Rice
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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Gabriella M. Wong
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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William J. Glassford
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
3Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, New York, USA
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Kayla I. Downs
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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Jessica L. Shastay
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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Kenechukwu Charles-Obi
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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Malini Natarajan
2Stowers Institute for Medical Research, Kansas City, Missouri, USA
4Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island, USA
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Madelaine Gogol
2Stowers Institute for Medical Research, Kansas City, Missouri, USA
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Julia Zeitlinger
2Stowers Institute for Medical Research, Kansas City, Missouri, USA
5Department of Pathology and Laboratory Medicine, Kansas University Medical Center, Kansas City, Kansas, USA
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Mark Rebeiz
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
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  • For correspondence: rebeiz@pitt.edu
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Abstract

During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have found network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the terminalia is highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (flygenitalia.pitt.edu) where they can serve as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 26, 2019.
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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia
Ben J. Vincent, Gavin R. Rice, Gabriella M. Wong, William J. Glassford, Kayla I. Downs, Jessica L. Shastay, Kenechukwu Charles-Obi, Malini Natarajan, Madelaine Gogol, Julia Zeitlinger, Mark Rebeiz
bioRxiv 677260; doi: https://doi.org/10.1101/677260
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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia
Ben J. Vincent, Gavin R. Rice, Gabriella M. Wong, William J. Glassford, Kayla I. Downs, Jessica L. Shastay, Kenechukwu Charles-Obi, Malini Natarajan, Madelaine Gogol, Julia Zeitlinger, Mark Rebeiz
bioRxiv 677260; doi: https://doi.org/10.1101/677260

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