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Aequorea victoria’s secrets

View ORCID ProfileGerard G. Lambert, View ORCID ProfileHadrien Depernet, View ORCID ProfileGuillaume Gotthard, View ORCID ProfileDarrin T. Schultz, View ORCID ProfileIsabelle Navizet, View ORCID ProfileTalley Lambert, Daphne S. Bindels, Vincent Levesque, View ORCID ProfileJennifer N. Moffatt, View ORCID ProfileAnya Salih, View ORCID ProfileAntoine Royant, Nathan C. Shaner
doi: https://doi.org/10.1101/677344
Gerard G. Lambert
Dept. of Neurosciences, Ctr. for Research in Biological Systems, University of California San Diego School of Medicine, La Jolla, CA USA
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  • ORCID record for Gerard G. Lambert
Hadrien Depernet
European Synchrotron Radiation Facility, Grenoble, France
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  • ORCID record for Hadrien Depernet
Guillaume Gotthard
European Synchrotron Radiation Facility, Grenoble, France
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  • ORCID record for Guillaume Gotthard
Darrin T. Schultz
University of California Santa Cruz, Santa Cruz, CA USAMonterey Bay Aquarium Research Institute, Moss Landing, CA USA
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  • ORCID record for Darrin T. Schultz
Isabelle Navizet
Université Paris-Est, Laboratoire Modélisation et Simulation Multi Échelle, MSME UMR 8208 CNRS, UPEM, Marne-la-Vallée, France
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Talley Lambert
Department of Cell Biology, Harvard Medical School, Boston, MA USADepartment of Systems Biology, Harvard Medical School, Boston, MA USA
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Daphne S. Bindels
Nikon Imaging Center, University of California San Diego, La Jolla, CA USA
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Vincent Levesque
Birch Aquarium at Scripps, La Jolla, CA USA
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Jennifer N. Moffatt
Confocal Facility, Western Sydney University, NSW, Australia
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  • ORCID record for Jennifer N. Moffatt
Anya Salih
Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale), Grenoble, France
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Antoine Royant
European Synchrotron Radiation Facility, Grenoble, FranceUniv. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale), Grenoble, France
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Nathan C. Shaner
Dept. of Neurosciences, Ctr. for Research in Biological Systems, University of California San Diego School of Medicine, La Jolla, CA USA
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  • For correspondence: ncshaner@ucsd.edu
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Abstract

Using mRNA-Seq and de novo transcriptome assembly, we identified, cloned and characterized nine previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from avGFP. Among these FPs are the brightest GFP homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including two that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 19, 2019.
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Aequorea victoria’s secrets
Gerard G. Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T. Schultz, Isabelle Navizet, Talley Lambert, Daphne S. Bindels, Vincent Levesque, Jennifer N. Moffatt, Anya Salih, Antoine Royant, Nathan C. Shaner
bioRxiv 677344; doi: https://doi.org/10.1101/677344
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Aequorea victoria’s secrets
Gerard G. Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T. Schultz, Isabelle Navizet, Talley Lambert, Daphne S. Bindels, Vincent Levesque, Jennifer N. Moffatt, Anya Salih, Antoine Royant, Nathan C. Shaner
bioRxiv 677344; doi: https://doi.org/10.1101/677344

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