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Chemogenetic Control of Nanobodies

View ORCID ProfileHelen Farrants, View ORCID ProfileMiroslaw Tarnawski, View ORCID ProfileThorsten G. Müller, View ORCID ProfileShotaro Otsuka, View ORCID ProfileJulien Hiblot, Birgit Koch, Moritz Kueblbeck, View ORCID ProfileHans-Georg Kräusslich, View ORCID ProfileJan Ellenberg, View ORCID ProfileKai Johnsson
doi: https://doi.org/10.1101/683557
Helen Farrants
1Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
2Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland
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Miroslaw Tarnawski
3Protein Expression and Characterization Facility, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
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Thorsten G. Müller
4Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany
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Shotaro Otsuka
5Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany
6Max Perutz Labs, a joint venture of the University of Vienna and the Medical University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria
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Julien Hiblot
1Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
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Birgit Koch
1Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
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Moritz Kueblbeck
5Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany
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Hans-Georg Kräusslich
4Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany
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Jan Ellenberg
5Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany
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Kai Johnsson
1Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
2Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland
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  • For correspondence: johnsson@mr.mpg.de
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ABSTRACT

We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted June 27, 2019.
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Chemogenetic Control of Nanobodies
Helen Farrants, Miroslaw Tarnawski, Thorsten G. Müller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kräusslich, Jan Ellenberg, Kai Johnsson
bioRxiv 683557; doi: https://doi.org/10.1101/683557
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Chemogenetic Control of Nanobodies
Helen Farrants, Miroslaw Tarnawski, Thorsten G. Müller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kräusslich, Jan Ellenberg, Kai Johnsson
bioRxiv 683557; doi: https://doi.org/10.1101/683557

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