Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

First Attempt to Identify and Map QTLs Associated with Promiscuous Nodulation in Soybean

Eric E. Agoyi, John B. Tumuhairwe, Godfree Chigeza, Phinehas Tukamuhabwa, Brian W. Diers
doi: https://doi.org/10.1101/688028
Eric E. Agoyi
1Department of Crop Science, University of Illinois, 1101 W Peabody Drive, Urbana, IL, 61801, USA
2Department of Agricultural Production, Makerere University, P.O. Box 7062, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ericagoyi@gmail.com
John B. Tumuhairwe
2Department of Agricultural Production, Makerere University, P.O. Box 7062, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Godfree Chigeza
3International Institute of Tropical Agriculture, IITA-Zambia, Plot 32, Poplar Avenue, Avondale, Lusaka, Zambia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Phinehas Tukamuhabwa
2Department of Agricultural Production, Makerere University, P.O. Box 7062, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian W. Diers
1Department of Crop Science, University of Illinois, 1101 W Peabody Drive, Urbana, IL, 61801, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

To inform possibility of conducting marker assisted breeding of promiscuous soybean varieties, this study used 92 F2 lines from biparental cross to identify QTLs associated with promiscuous nodulation in soybean. GBS; genotyping by sequencing platform was used to generate SNPs through the pipeline 2 in TASSEL 5.0, Bowtie2 version 2.2.8 for tag alignment, Beagle version 4.1 to impute missing SNPs and R-QTL package in R for QTL identification. Four nodulation traits were assessed viz number of nodules (NN), percent of effective nodules (NE), fresh weight and dry weight of nodules (NFW and NDW). Two QTLs were identified on chromosomes 10 and 13. Both QTLs were associated with NN, only QTL13 was associated NE and only QTL10 was associated with nodule ‘weights. It was observed that NN, NFW and NDW shared QTL10 and NN and NE share QTL13 allowing hypothetize on the existence of pleiotropic genes in those those two regions. Over dominance effect was observed for QTL10 and non additive effect for QTL13. The paper recommend investigations be pursued to validate those QTLs and set foundation for marker assisted selection of promiscuous soybean varieties. Also these findings could serve as starting point for gene cloning to better understand nodulation trait in soybean.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted July 01, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
First Attempt to Identify and Map QTLs Associated with Promiscuous Nodulation in Soybean
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
First Attempt to Identify and Map QTLs Associated with Promiscuous Nodulation in Soybean
Eric E. Agoyi, John B. Tumuhairwe, Godfree Chigeza, Phinehas Tukamuhabwa, Brian W. Diers
bioRxiv 688028; doi: https://doi.org/10.1101/688028
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
First Attempt to Identify and Map QTLs Associated with Promiscuous Nodulation in Soybean
Eric E. Agoyi, John B. Tumuhairwe, Godfree Chigeza, Phinehas Tukamuhabwa, Brian W. Diers
bioRxiv 688028; doi: https://doi.org/10.1101/688028

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3497)
  • Biochemistry (7341)
  • Bioengineering (5317)
  • Bioinformatics (20248)
  • Biophysics (9999)
  • Cancer Biology (7734)
  • Cell Biology (11291)
  • Clinical Trials (138)
  • Developmental Biology (6431)
  • Ecology (9943)
  • Epidemiology (2065)
  • Evolutionary Biology (13311)
  • Genetics (9358)
  • Genomics (12575)
  • Immunology (7696)
  • Microbiology (18998)
  • Molecular Biology (7432)
  • Neuroscience (40971)
  • Paleontology (300)
  • Pathology (1228)
  • Pharmacology and Toxicology (2133)
  • Physiology (3154)
  • Plant Biology (6855)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1895)
  • Systems Biology (5309)
  • Zoology (1087)