Abstract
Motivation Metagenomic and metatranscriptomic sequencing analyses have become increasingly popular tools for producing massive amounts of short-read data, often used for the reconstruction of draft genomes or the detection of (active) genes in microbial communities. Unfortunately, sequence assemblies of such datasets generally remain a computationally challenging task. Frequently, researchers are only interested in a specific group of organisms or genes; yet, the assembly of multiple datasets only to identify candidate sequences for a specific question is sometimes prohibitively slow, forcing researchers to select a subset of available datasets to address their question. Here we present PhyloMagnet, a workflow to screen meta-omics datasets for taxa and genes of interest using gene-centric assembly and phylogenetic placement of sequences.
Results Using PhyloMagnet, we could identify up to 87% of the genera in an in vitro mock community with variable abundances, while the false positive predictions per single gene tree ranged from 0% to 23%. When applied to a group of metagenomes for which a set of MAGs have been published, we could detect the majority of the taxonomic labels that the MAGs had been annotated with. In a metatranscriptomic setting the phylogenetic placement of assembled contigs corresponds to that of transcripts obtained from transcriptome assembly.
Availability PhyloMagnet is built using Nextflow, available at github.com/maxemil/PhyloMagnet and is developed and tested on Linux. It is released under the open source GNU GPL license and documentation is available at phylomagnet.readthedocs.io. Version 0.5 of PhyloMagnet was used for all benchmarks experiments.
Footnotes
correct author name
