Abstract
Background Genetically encoded biosensors are useful tools for the detection of metabolites and industrially valuable molecules, and present many potential applications in biotechnology and biomedicine. However, the most common approach to develop biosensors relies on employing a limited set of naturally occurring allosteric transcript factors (aTFs). Therefore, altering the substrate specificity of aTFs towards the detection of new effectors is an important goal.
Results Here, the PcaV repressor, a member of the MarR aTF family, was used to develop a biosensor for the detection of hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA). The PCA biosensor was further subjected to directed evolution to alter its substrate specificity towards vanillin and other closely related aromatic aldehydes, to generate the Van2 biosensor. Substrate recognition of Van2 was explored in vitro using a range of biochemical and biophysical analyses, and extensive in vivo genetic-phenotypic analysis was performed to determine the role of each amino acid change upon biosensor performance.
Conclusions This is the first study to report directed evolution of a member of the MarR aTF family, and demonstrates the plasticity of the PCA biosensor by altering its substrate specificity to generate a biosensor for aromatic aldehydes.
List of abbreviations
- aTF
- allosteric transcription factor
- PCA
- protocatechuic acid
- EBD
- effector binding domain
- DBD
- DNA binding domain
- RNAP
- RNA polymerase
- DMSO
- dimethyl sulfoxide
- RFU
- relative fluorescence unit
- OE-PCR
- overlap-extension polymerase chain reaction
- DE
- directed evolution
- FACS
- fluorescence activated cell sorting
- MTBE
- methyl-tert-butyl ether
- EMSA
- electrophoretic mobility shift assay
- SEC-MALS
- size-exclusion chromatography coupled to multi-angle light scattering
- SPR
- Surface Plasmon Resonance